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Invitrogen
DIO1 Polyclonal Antibody
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FIGURE: 1 / 6
DIO1 Antibody (PA5-100139) in ICC/IF
Immunofluorescent analysis of DIO1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DIO1 polyclonal antibody (Product # PA5-100139) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100139
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DIO1(Accession P49895), corresponding to amino acid residues Q220-S249.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total DIO1.
Target Information
The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some, but not all, isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5DI; deiodinase, iodothyronine type I; DIOI; hDido1; iodothyronine deiodinase type 1; selenoprotein DIO1; thyroxine 5'-deiodinase; thyroxine deiodinase; thyroxine deiodinase type I (selenoprotein); thyroxine deiodinase, type I; Type 1 DI; Type I iodothyronine deiodinase; Type-I 5'-deiodinase; unnamed protein product
Gene Aliases: 5DI; D1; DIO1; ITDI1; THMA2; TXDI1
UniProt ID: (Human) Q3KNP8, (Mouse) Q61153, (Rat) P24389
Entrez Gene ID: (Human) 1733, (Mouse) 13370, (Rat) 25430
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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