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DIO2 Polyclonal Antibody
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FIGURE: 1 / 4
DIO2 Antibody (PA5-18659) in ICC/IF
Immunocytochemistry analysis of DIO2 using DIO2 Polyclonal Antibody (Product # PA5-18659) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18659
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (EVKKHQNQEDRC) corresponding to the internal amino acids of DIO2 (aa 181-192).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with human, porcine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
Target Information
The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine by outer ring deiodination to bioactive 3,3',5-triiodothyronine residues encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5DII; deiodinase, iodothyronine type II; deiodinase-2; deiodonase-2; DIOII; iodothyronine type II deiodinase; selenoprotein Y; thyroxine deiodinase; thyroxine deiodinase, type II; thyroxine type II; Type 2 DI; type 2 iodothyronine deiodinase; Type II iodothyronine deiodinase; type-II 5' deiodinase; Type-II 5'-deiodinase; type-II 5'deiodinase; unnamed protein product
Gene Aliases: 5DII; AI324267; D2; DIO2; DIOII; ITDI2; SELENOY; SelY; TXDI2
UniProt ID: (Human) Q92813, (Mouse) Q9Z1Y9
Entrez Gene ID: (Human) 1734, (Mouse) 13371
selenocysteine incorporation
thyroid hormone generation
response to lipopolysaccharide
thyroid hormone metabolic process
thyroid hormone catabolic process
hormone biosynthetic process
positive regulation of cold-induced thermogenesis
response to cold
oxidation-reduction process
thyroid-stimulating hormone secretion
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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