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Immunofluorescence analysis of DISC1 (C-TERM) was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with DISC1 (C-TERM) Rabbit Polyclonal Antibody (406800) at 1ug/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Non-human primate, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of the mouse DISC1 (disrupted in schizophrenia 1) protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
DISC1 (Disrupted in Schizophrenia 1) is a multifunctional protein that interacts with multiple proteins of the centrosome and cytoskeletal system, and is involved in neuronal functions that are dependent upon proper cytoskeletal regulation, including neuronal migration, neurite architecture and intracellular transport. Regions of the brain which express DISC1 include the hippocampus, lateral septum, amygdala, cerebral cortex, cerebellum and paraventricular hypothalamus, and loss of DISC1 function results in differences in structural organization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Synergistic interactions between PDE4B and GSK-3: DISC1 mutant mice.||Lipina TV,Wang M,Liu F,Roder JC||Neuropharmacology (62:1252)||2012|
|Mouse||Not Cited||Synergistic interactions between PDE4B and GSK-3: DISC1 mutant mice.||Lipina TV,Wang M,Liu F,Roder JC||Neuropharmacology (62:1252)||2012|
|Mouse||Not Cited||Genetic and pharmacological evidence for schizophrenia-related Disc1 interaction with GSK-3.||Lipina TV,Kaidanovich-Beilin O,Patel S,Wang M,Clapcote SJ,Liu F,Woodgett JR,Roder JC||Synapse (New York, N.Y.) (65:234)||2011|
|DISC1 regulates primary cilia that display specific dopamine receptors.||Marley A,von Zastrow M||PloS one (5:null)||2010|
||Genetic and pharmacological evidence for schizophrenia-related Disc1 interaction with GSK-3.||Lipina TV,Kaidanovich-Beilin O,Patel S,Wang M,Clapcote SJ,Liu F,Woodgett JR,Roder JC||Synapse (New York, N.Y.) (65:234)||2011|
|The von Economo neurons in frontoinsular and anterior cingulate cortex in great apes and humans.||Allman JM,Tetreault NA,Hakeem AY,Manaye KF,Semendeferi K,Erwin JM,Park S,Goubert V,Hof PR||Brain structure & function (214:495)||2010|
C1orf136; Disrupted in schizophrenia 1 homolog; Disrupted in schizophrenia 1 protein; RP4-730B13.1; SCZD9
C1orf136; DISC1; KIAA0457; SCZD9