|Immunohistochemistry (Frozen) (IHC (F))||2.5-5.0 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2.5-5 µg/ml|
|Western Blot (WB)||1:100-1:2000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal region (within residues 400-500) of the rat DISC1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||sodium borate|
|Storage conditions||4° C, store in dark|
The theoretical molecular weight of DISC1 is ~90 kDa.
Due to alternative splicing, DISC1 may also run at ~75 and 100 kDa.
Suggested positive control: human brain sections (neurons and glia).
The 'Disrupted In Schizophrenia' gene locus DISC is associated with patients afflicted with schizophrenia as a result of chromosomal translocations. DISC-1 encodes a large protein predicted to contain a globular N-terminal domain and a helical C-terminal domain, both of which have the potential to form interactions with other proteins. DISC-1 interacts with proteins involved in the centrosome and cytoskeletal system, including MIPT3, MAP1A and NUDEL; proteins which localize receptors to membranes, including alpha-actinin 2 an beta 4-spectrin; and proteins which transduce signals from membrane receptors, including ATF-4 and ATF-5. Therefore, DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain.
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Protein Aliases: C1orf136; Disrupted in schizophrenia 1 homolog; Disrupted in schizophrenia 1 protein; RP4-730B13.1; SCZD9
Gene Aliases: C1orf136; DISC1; KIAA0457; SCZD9
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