This antibody is predicted to react with bovine, chicken, canine, mouse, porcine and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is normal breast tissue.
DJ-1 protein has 189 amino acids and was cloned from a HeLa cell cDNA library as a novel oncogene which weakly transformed NIH3T3 cells. The expression of DJ-1 is induced by growth signals and is translocated from the cytoplasm to the nucleus during the S phase, although no nuclear localization signals are present. It has been suggested that DJ-1 is a mitogen-dependent oncogene product involved in Ras-related signal transduction pathway.
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Protein Aliases: DJ-1; DJ1; early onset 7; epididymis secretory sperm binding protein Li 67p; Maillard deglycase; Oncogene DJ1; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease autosomal recessive; Parkinson disease protein 7; parkinson protein 7; Parkinsonism-associated deglycase; Protein DJ-1; Protein/nucleic acid deglycase DJ-1
Gene Aliases: DJ-1; DJ1; HEL-S-67p; PARK7
UniProt ID: (Human) Q99497
Entrez Gene ID: (Human) 11315