|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human DJ-1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is normal breast tissue.
DJ-1 protein has 189 amino acids and was cloned from a HeLa cell cDNA library as a novel oncogene which weakly transformed NIH3T3 cells. The expression of DJ-1 is induced by growth signals and is translocated from the cytoplasm to the nucleus during the S phase, although no nuclear localization signals are present. It has been suggested that DJ-1 is a mitogen-dependent oncogene product involved in Ras-related signal transduction pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.