Proteintech

DKK1 Polyclonal Antibody

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DKK1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HepG2 cells, using DKK1 antibody 21112-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DKK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:200 (under 40x lens).

DKK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 21112-1-AP ( DKK1 Antibody) at dilution of 1:200 (under 10x lens).

DKK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 21112-1-AP ( DKK1 Antibody) at dilution of 1:200 (under 40x lens).

DKK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:50 (under 10x lens).

DKK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:50 (under 40x lens).

DKK1 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DKK1 Antibody in Western Blot (WB) — Sp2/0 cells were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DKK1 Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

DKK1 Antibody in Western Blot (WB) — Sp2/0 cells were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DKK1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DKK1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DKK1 Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:10-1:100

-

Immunofluorescence (IF)

1:10-1:100

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Western Blot (WB)

1:500-1:1000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DKK1 Fusion Protein Ag15110

Conjugate

Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: dickkopf 1 homolog; dickkopf homolog 1; dickkopf related protein-1; Dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; Dickkopf-related protein 1; Dickkopf1; dkk-1; hDkk-1; hDkk1; SK

Gene Aliases: DKK-1; DKK1; mdkk-1; SK; UNQ492/PRO1008

UniProt ID: (Human) O94907, (Mouse) O54908

Entrez Gene ID: (Human) 22943, (Mouse) 13380, (Rat) 293897

negative regulation of transcription from RNA polymerase II promoter cell morphogenesis involved in differentiation endoderm formation mesoderm formation hair follicle development regulation of receptor internalization negative regulation of Wnt signaling pathway embryonic limb morphogenesis negative regulation of BMP signaling pathway forebrain development negative regulation of protein binding response to retinoic acid negative regulation of peptidyl-serine phosphorylation negative regulation of mesodermal cell fate specification regulation of endodermal cell fate specification negative regulation of skeletal muscle tissue development negative regulation of synapse assembly regulation of synaptic transmission, glutamatergic face morphogenesis negative regulation of pathway-restricted SMAD protein phosphorylation motor learning positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway negative regulation of canonical Wnt signaling pathway Wnt signaling pathway involved in somitogenesis extracellular negative regulation of signal transduction negative regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment regulation of dopaminergic neuron differentiation midbrain morphogenesis negative regulation of Wnt-Frizzled-LRP5/6 complex assembly negative regulation of cardiac muscle cell differentiation multicellular organism development endoderm development Wnt signaling pathway regulation of Wnt signaling pathway negative regulation of protein complex assembly head morphogenesis negative regulation of receptor activity negative regulation of ossification

Process(es)

signal transducer activity protein binding growth factor activity co-receptor binding receptor antagonist activity low-density lipoprotein particle receptor binding molecular_function