Proteintech

DKK1 Polyclonal Antibody

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DKK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using DKK1 antibody 21112-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DKK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:200 (under 40x lens).

DKK1 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

21112-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 7 publications 7 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DKK1 Fusion Protein Ag15110 (32-191 aa encoded by BC001539) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: TLNSVLNSN AIKNLPPPLG GAAGHPGSAV SAAPGILYPG GNKYQTIDNY QPYPCAEDEE CGTDEYCASP TRGGDAGVQI CLACRKRRKR CMRHAMCCPG NYCKNGICVS SDQNHFRGEI EETITESFGN DHSTLDGYSR RTTLSSKMYH TKGQEGSVCL R (32-191 aa encoded by BC001539)

Target Information

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Mature human DKK-1 expressed in HEK293 cells is a 35-40 kDa glycoprotein containing 235 amino acid residues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Chinese medicine

Wang Q,Zang W,Han L,Yang L,Ye S,Ouyang J,Zhang C,Bi Y,Zhang C,Bian H

Fri Apr 08 00:00:00 EDT 2022

BPI-9016M, a c-Met inhibitor, suppresses tumor cell growth, migration and invasion of lung adenocarcinoma via miR203-DKK1.

Theranostics

Zhang P,Li S,Lv C,Si J,Xiong Y,Ding L,Ma Y,Yang Y

Thu Sep 05 00:00:00 EDT 2019

BPI-9016M, a c-Met inhibitor, suppresses tumor cell growth, migration and invasion of lung adenocarcinoma via miR203-DKK1.

Theranostics

Zhang P,Li S,Lv C,Si J,Xiong Y,Ding L,Ma Y,Yang Y

Thu Sep 05 00:00:00 EDT 2019

Distinctive role of ACVR1 in dentin formation: requirement for dentin thickness in molars and prevention of osteodentin formation in incisors of mice.

Journal of molecular histology

Zhang X,Shi C,Zhao H,Zhou Y,Hu Y,Yan G,Liu C,Li D,Hao X,Mishina Y,Liu Q,Sun H

Fri Feb 01 00:00:00 EST 2019

Rehmanniae Radix Preparata suppresses bone loss and increases bone strength through interfering with canonical Wnt/β-catenin signaling pathway in OVX rats.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA

Liu C,Wang L,Zhu R,Liu H,Ma R,Chen B,Li L,Guo Y,Jia Q,Shi S,Zhao D,Mo F,Zhao B,Niu J,Fu M,Orekhov AN,Brömme D,Gao S,Zhang D

Fri Feb 01 00:00:00 EST 2019

Radix Salviae miltiorrhizae improves bone microstructure and strength through Wnt/β-catenin and osteoprotegerin/receptor activator for nuclear factor-κB ligand/cathepsin K signaling in ovariectomized rats.

Phytotherapy research : PTR

Liu H,Zhu R,Wang L,Liu C,Ma R,Qi B,Chen B,Li L,Guo Y,Shi S,Jia Q,Niu J,Zhao D,Mo F,Gao S,Zhang D

Sat Dec 01 00:00:00 EST 2018

Apobec-1 complementation factor regulates cell migration and apoptosis through Dickkopf1 by acting on its 3' untranslated region in MCF7 cells.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Yan X,Li Q,Ni D,Xie Y,He Q,Wan Q,Liu Y,Lyu Z,Mao Z,Zhou Q

Thu Jun 01 00:00:00 EDT 2017

MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C (RhoC).

Oncotarget

Liu BL,Sun KX,Zong ZH,Chen S,Zhao Y

Tue Feb 09 00:00:00 EST 2016

Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.

PloS one

Ying Q,Ansong E,Diamond AM,Lu Z,Yang W,Bie X

Thu Feb 04 00:00:00 EST 2016

Bioinformatics

Protein Aliases: dickkopf 1 homolog; dickkopf homolog 1; dickkopf related protein-1; Dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; Dickkopf-related protein 1; Dickkopf1; dkk-1; hDkk-1; hDkk1; SK

Gene Aliases: DKK-1; DKK1; mdkk-1; SK; UNQ492/PRO1008

UniProt ID: (Human) O94907, (Mouse) O54908

Entrez Gene ID: (Human) 22943, (Mouse) 13380, (Rat) 293897

Function(s)