Invitrogen

DLD Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Tech Support

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DLD Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DLD in U-251 MG cells using a DLD polyclonal antibody (Product # PA5-83742). The analysis shows localization to mitochondria.

DLD Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of DLD in human kidney and epididymis tissues using a DLD polyclonal antibody (Product # PA5-83742). Corresponding RNA-seq data are presented for the same tissues.

DLD Antibody in Western Blot (WB) — Western blot analysis of DLD by a DLD polyclonal antibody (Product # PA5-83742). Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human plasma (IgG/HSA depleted) Lane 5: Human liver tissue Lane 6: Human tonsil tissue.

DLD Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of DLD demonstrates antibody specificity. Immunohistochemical analysis of DLD using anti-DLD Polyclonal Antibody (Product #PA5-83742), shows significant staining of DLD in kidney and shows minimal or weak staining in epididymis tissues. The relative expression levels of DLD within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-83742

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:1,000-1:2,500	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human DLD View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2790894

Product Specific Information

Immunogen sequence: DVVAGPMLAH KAEDEGIICV EGMAGGAVHI DYNCVPSVIY THPEVAWVGK SEEQLKEEGI EYKVGKFPFA ANSRAKTNAD TDGMVK

Target Information

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: diaphorase; Dihydrolipoamide dehydrogenase; Dihydrolipoyl dehydrogenase, mitochondrial; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; Glycine cleavage system L protein; glycine cleavage system protein L; lipoamide dehydrogenase; lipoamide reductase; lipoyl dehydrogenase; OTTHUMP00000206744; OTTHUMP00000206746; OTTHUMP00000206748; OTTHUMP00000206749; PHE 3

Gene Aliases: DLD; DLDD; DLDH; E3; GCSL; LAD; PHE3

UniProt ID: (Human) P09622

Entrez Gene ID: (Human) 1738

Molecular Function: oxidoreductase