Invitrogen

DLK1 Polyclonal Antibody

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DLK1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry of DLK1 in HepG2 cells with DLK1 Polyclonal Antibody (Product # PA5-34433) at 2.5 µg/mL.

DLK1 Antibody in Western Blot (WB) — Western blot was performed using Anti-DLK1 Polyclonal Antibody (Product # PA5-34433) and a 36 kDa band corresponding to Protein delta homolog 1 was observed along with a strong uncharacterized band (*) around 70 kDa. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), SH-SY5Y (Lane 2), ACHN (Lane 3), T98G (Lane 4), 3T3-L1 (Lane 5) and 3T3-L1 differentiated to adipocytes (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL ) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20,000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). Band of interest was prominent in 3T3-L1, whereas it was absent in 3T3-L1 differentiated to adipocytes as expected (DOI 10.1074/jbc.M500463200). Expression was found to be null in ACHN (https://doi.org/10.1093/hmg/ddl001) and T98G (https://doi.org/10.1038/sj.onc.1209219) as well in accordance with the reports.

Product Details

PA5-34433

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunocytochemistry (ICC/IF)	25 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	An 18 amino acid synthetic peptide near the carboxy terminus of human DLK1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
RRID	AB_2551785

Product Specific Information

A suggested positive control is HepG2 cell lysate.

PA5-34433 can be used with blocking peptide PEP-1475.

Target Information

The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Adipocyte differentiation inhibitor protein; delta-like 1 homolog; DLK-1; FA1; Fetal antigen 1; pG2; Preadipocyte factor 1; preadipocyte factor-1; PREF; Pref-1; Protein delta homolog 1; secredeltin; stromal cell derived protein 1

Gene Aliases: AW742678; Delta1; DLK; DLK-1; DLK1; DlkI; FA1; Ly107; Peg9; pG2; Pref-1; PREF1; Scp-1; SCP1; ZOG

UniProt ID: (Human) P80370, (Mouse) Q09163, (Rat) O70534

Entrez Gene ID: (Human) 8788, (Mouse) 13386, (Rat) 114587

Molecular Function: membrane-bound signaling molecule