Invitrogen

DLL1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (21) DLL1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)
Advanced Verification (3)

FIGURE: 1 / 7

DLL1 Antibody (PA5-19106) in WB

Western blot was performed using Anti-DLL1 Polyclonal Antibody (Product # PA5-19106) and an 80 kDa band corresponding to DLL1 was observed across tissues. Tissue extracts (50 µg lysate) of Mouse Heart (Lane 1), Mouse Spleen (Lane 2), Rat Heart (Lane 3), Rat Spleen (Lane 4) or Mouse Testis (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed... View More

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DLL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, using a Polyclonal DLL1 Antibody (Product # PA5-19106). Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

DLL1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of DLL1 in HeLa cells using a polyclonal antibody (Product #PA5-19106). HeLa cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

DLL1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Mouse Heart, Mouse Spleen, Rat Heart, Rat Spleen, Mouse Testis owing to their inherent genetic constitution. Relative expression of DLL1 was observed in Mouse and Rat Heart and Spleen as compared to Mouse Testis using Anti-DLL1 Polyclonal Antibody (Product # PA5-19106) in Western Blot. {RE}

Product Details

PA5-19106

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-7 µg/mL

Immunocytochemistry (ICC/IF)

10 µg/mL

Flow Cytometry (Flow)

10 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (ATQRHLTVGEEWSQD) corresponding to the internal amino acids of DLL1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10977414

Product Specific Information

This antibody is predicted to react with human based on sequence homology.

Target Information

Delta-like protein 1 (DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: delta like-1; Delta-like protein 1; Delta1; Dll1-derived cell-associated form; Dll1-EC; Dll1-IC; Dll1-intracellular form; Dll1-soluble form; Dll1-TMIC; Drosophila Delta homolog 1; H-Delta-1; OTTHUMP00000017690

Gene Aliases: Delta; DELTA1; DL1; DLL1; UNQ146/PRO172

UniProt ID: (Human) O00548, (Mouse) Q61483, (Rat) P97677

Entrez Gene ID: (Human) 28514, (Mouse) 13388, (Rat) 84010

Function(s)

Process(es)

Notch binding calcium ion binding protein binding Tat protein binding scaffold protein binding