Invitrogen

DLL1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (22) DLL1 antibodies

Questions & Answers

Questions & Answers

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DLL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, using a Polyclonal DLL1 Antibody (Product # PA5-19106). Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

DLL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-DLL1 Polyclonal Antibody (Product # PA5-19106) and an 80 kDa band corresponding to DLL1 was observed across tissues. Tissue extracts (50 µg lysate) of Mouse Heart (Lane 1), Mouse Spleen (Lane 2), Rat Heart (Lane 3), Rat Spleen (Lane 4) or Mouse Testis (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).

DLL1 Antibody in Western Blot (WB) — Western Blot staining of Rat Heart lysate using Product # PA5-19106 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

DLL1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of DLL1 in HeLa cells using a polyclonal antibody (Product #PA5-19106). HeLa cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

DLL1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Mouse Heart, Mouse Spleen, Rat Heart, Rat Spleen, Mouse Testis owing to their inherent genetic constitution. Relative expression of DLL1 was observed in Mouse and Rat Heart and Spleen as compared to Mouse Testis using Anti-DLL1 Polyclonal Antibody (Product # PA5-19106) in Western Blot. {RE}

DLL1 Antibody — Dll1-dependent Notch1 signaling contributes to cisplatin-induced renal tubular cell death. A and B, Western blot images and bar graphs (n = 3) confirming Dll1 knockdown in Dll1 siRNA-transfected HK-2 cells. C, bar graphs summarizing the levels of cleaved Notch1 in scrambled (control; Scrm) siRNA- and Dll1 siRNA-transfected HK-2 cells (n = 6 each). D, kinetic curves (n = 12 each) demonstrating that cisplatin (30 µmol L-1)-induced caspase-3/7 activity in HK-2 is diminished in Dll1 siRNA-transfected HK-2 cells. $ P < 0.05 vs Scrm siRNA; # P < 0.05 vs Scrm siRNA + cisplatin (8-22 h for D); *P < 0.05 vs Scrm and Dll1 siRNA + Captisol (8-22 h) {KD}

DLL1 Antibody — Figure 2 Cisplatin induces Notch signaling in mouse kidneys. A-D, Western blot images and bar graphs showing Dll1 and Jag1 protein expression levels in the kidneys of mice 5 days after treatment with Captisol (vehicle control; IP ) and cisplatin (15 mg/kg; IP ). E and F, Western blot images and bar graphs illustrating N1 ICD protein expression levels in the kidneys of Captisol-, cisplatin-, and DAPT (15 mg/kg; IP ) + cisplatin-treated mice. * P < 0.05 vs Captisol; # P < 0.05 vs cisplatin; n = 4 each {TM}

Product Details

PA5-19106

Applications	Tested Dilution	Publications
Western Blot (WB)	0.3-7 µg/mL	-
Immunocytochemistry (ICC/IF)	10 µg/mL	-
Flow Cytometry (Flow)	10 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (ATQRHLTVGEEWSQD) corresponding to the internal amino acids of DLL1
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10977414

Product Specific Information

This antibody is predicted to react with human based on sequence homology.

Target Information

Delta-like protein 1 (Dlk1, DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. DLL1, which is also known as preadipocyte factor 1 (pref-1) because of its ability to inhibit preadipocyte differentiation, regulates the differentiation of several other cell types. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: delta like-1; Delta-like protein 1; Delta1; Dll1-derived cell-associated form; Dll1-EC; Dll1-IC; Dll1-intracellular form; Dll1-soluble form; Dll1-TMIC; Drosophila Delta homolog 1; H-Delta-1; OTTHUMP00000017690

Gene Aliases: Delta; DELTA1; DL1; DLL1; UNQ146/PRO172

UniProt ID: (Human) O00548, (Mouse) Q61483, (Rat) P97677

Entrez Gene ID: (Human) 28514, (Mouse) 13388, (Rat) 84010

in utero embryonic development somitogenesis somite specification heart looping cell communication Notch signaling pathway cell-cell signaling multicellular organism development determination of left/right symmetry compartment pattern specification nervous system development organ morphogenesis auditory receptor cell fate commitment cell differentiation negative regulation of interleukin-10 production inner ear morphogenesis odontogenesis of dentin-containing tooth auditory receptor cell differentiation negative regulation of cell differentiation negative regulation of auditory receptor cell differentiation negative regulation of myeloid cell differentiation negative regulation of neuron differentiation positive regulation of Notch signaling pathway positive regulation of transcription from RNA polymerase II promoter inner ear development regulation of neurogenesis Notch signaling involved in heart development left/right axis specification cell fate determination proximal tubule development loop of Henle development neuronal stem cell population maintenance marginal zone B cell differentiation type B pancreatic cell development Notch receptor processing regulation of blood pressure positive regulation of cell proliferation negative regulation of cell proliferation proximal/distal pattern formation astrocyte development regulation of somitogenesis spinal cord development cerebellar molecular layer formation cerebellar Purkinje cell layer structural organization hemopoiesis regulation of cell adhesion negative regulation of epithelial cell differentiation negative regulation of glial cell apoptotic process organ growth regulation of growth negative regulation of epidermal cell differentiation negative regulation of myoblast differentiation positive regulation of endocytosis lateral inhibition skeletal muscle tissue growth regulation of skeletal muscle tissue growth positive regulation of skeletal muscle tissue growth neuron fate specification regulation of cell division retina development in camera-type eye retina morphogenesis in camera-type eye Notch signaling pathway involved in arterial endothelial cell fate commitment nephron development clathrin-mediated endocytosis endothelial tip cell fate specification skin epidermis development regulation of vascular endothelial growth factor signaling pathway positive regulation of sprouting angiogenesis regulation of energy homeostasis

Process(es)

Notch binding calcium ion binding protein binding Tat protein binding scaffold protein binding