Invitrogen

DLL1 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

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DLL1 Antibody in Western Blot (WB) — Western Blot staining of Rat Heart lysate using Product # PA5-19106 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

DLL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-DLL1 Polyclonal Antibody (Product # PA5-19106) and an 80 kDa band corresponding to DLL1 was observed across tissues. Tissue extracts (50 µg lysate) of Mouse Heart (Lane 1), Mouse Spleen (Lane 2), Rat Heart (Lane 3), Rat Spleen (Lane 4) or Mouse Testis (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).

DLL1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of DLL1 in HeLa cells using a polyclonal antibody (Product #PA5-19106). HeLa cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

DLL1 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across Mouse Heart, Mouse Spleen, Rat Heart, Rat Spleen, Mouse Testis owing to their inherent genetic constitution. Relative expression of DLL1 was observed in Mouse and Rat Heart and Spleen as compared to Mouse Testis using Anti-DLL1 Polyclonal Antibody (Product # PA5-19106) in Western Blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.3 µg/mL

-

Flow Cytometry (Flow)

10 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (ATQRHLTVGEEWSQD) corresponding to the internal amino acids of DLL1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10977414

Product Specific Information

This antibody is predicted to react with human based on sequence homology.

Target Information

Delta-like protein 1 (Dlk1, DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. DLL1, which is also known as preadipocyte factor 1 (pref-1) because of its ability to inhibit preadipocyte differentiation, regulates the differentiation of several other cell types. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: delta like-1; Delta-like protein 1; Delta1; Dll1-derived cell-associated form; Dll1-EC; Dll1-IC; Dll1-intracellular form; Dll1-soluble form; Dll1-TMIC; Drosophila Delta homolog 1; H-Delta-1; OTTHUMP00000017690

Gene Aliases: Delta; DELTA1; DL1; DLL1; UNQ146/PRO172

UniProt ID: (Human) O00548, (Mouse) Q61483, (Rat) P97677

Entrez Gene ID: (Human) 28514, (Mouse) 13388, (Rat) 84010

cell fate determination somitogenesis somite specification heart looping marginal zone B cell differentiation type B pancreatic cell development Notch signaling pathway Notch receptor processing determination of left/right symmetry compartment pattern specification regulation of blood pressure positive regulation of cell proliferation negative regulation of cell proliferation proximal/distal pattern formation astrocyte development regulation of somitogenesis spinal cord development cerebellar molecular layer formation cerebellar Purkinje cell layer structural organization hemopoiesis cell differentiation regulation of cell adhesion negative regulation of epithelial cell differentiation negative regulation of interleukin-10 production negative regulation of glial cell apoptotic process organ growth regulation of growth negative regulation of cell differentiation negative regulation of epidermal cell differentiation negative regulation of auditory receptor cell differentiation negative regulation of myeloid cell differentiation negative regulation of myoblast differentiation negative regulation of neuron differentiation positive regulation of Notch signaling pathway positive regulation of endocytosis positive regulation of transcription from RNA polymerase II promoter lateral inhibition skeletal muscle tissue growth regulation of skeletal muscle tissue growth positive regulation of skeletal muscle tissue growth neuron fate specification inner ear development regulation of neurogenesis regulation of cell division retina development in camera-type eye retina morphogenesis in camera-type eye Notch signaling pathway involved in arterial endothelial cell fate commitment left/right axis specification nephron development proximal tubule development loop of Henle development clathrin-mediated endocytosis endothelial tip cell fate specification neuronal stem cell population maintenance skin epidermis development regulation of vascular endothelial growth factor signaling pathway positive regulation of sprouting angiogenesis regulation of energy homeostasis in utero embryonic development cell communication cell-cell signaling multicellular organism development nervous system development organ morphogenesis auditory receptor cell fate commitment inner ear morphogenesis odontogenesis of dentin-containing tooth auditory receptor cell differentiation Notch signaling involved in heart development

Process(es)

Notch binding calcium ion binding protein binding Tat protein binding scaffold protein binding