Invitrogen

DLL1 Polyclonal Antibody

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View all (21) DLL1 antibodies

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Antibody Testing Data (3)
Published Figures (1)

DLL1 Antibody in Western Blot (WB)

FIGURE: 1 / 4

DLL1 Antibody (PA5-42902) in WB

Western blot was performed using Anti-DLL1 Polyclonal Antibody (Product # PA5-42902) and ~78kDa band corresponding to DLL1 was observed across all the cell lines and tissues tested. Membrane extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), PANC-1 (Lane 3), MCF7 (Lane 4), THP-1 (Lane 5), Mouse Spleen (Lane 6), Rat Spleen (Lane 7), Rat Heart (Lane 8) and Mouse Heart (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane ... View More

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DLL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-DLL1 Polyclonal Antibody (Product # PA5-42902) and ~78kDa band corresponding to DLL1 was observed across all the cell lines and tissues tested. Membrane extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), PANC-1 (Lane 3), MCF7 (Lane 4), THP-1 (Lane 5), Mouse Spleen (Lane 6), Rat Spleen (Lane 7), Rat Heart (Lane 8) and Mouse Heart (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

DLL1 Antibody in Western Blot (WB) — Western blot analysis of Hela cell lysate using an anti-DLL1 polyclonal antibody (Product # PA5-42902). Lane 1) Primary Antibody; Lane 2) Primary Antibody + Blocking Peptide.

DLL1 Antibody in Western Blot (WB) — Western blot analysis of human HeLa cell lysate using an anti-DLL1 polyclonal antibody (Product # PA5-42902).

DLL1 Antibody in Western Blot (WB) — Fig. 4 DLL1 overexpression improves the myogenic propensity of mesodermal progenitors. A Schematic overview of the doxycycline (dox)-inducible DLL1 construct flipped into an embryonic stem cell (ESC) master line. B Quantitative PCR for DLL1 after exposure of the generated ESC line to dox for two or four days. C Western blot for DLL1 and NOTCH1 after exposure of the generated ESC line to dox for four days. Tubulin (TUB) was used as a housekeeping protein. Quantification graph is on the right. D Immunofluorescence analysis of lamin A/C-positive mesodermal progenitors, with or without dox, (green) in co-culture with C2C12 myoblasts ( n = 3). Scale bars: 100 um. * P < 0.05.

Product Details

PA5-42902

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

5 µg/mL

-

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human DLL1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2610508

Product Specific Information

Peptide sequence: GSRCALALAV LSALLCQVWS SGVFELKLQE FVNKKGLLGN RNCCRGGAGP

Sequence homology: Cow: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%

Target Information

Delta-like protein 1 (DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Valproic acid stimulates myogenesis in pluripotent stem cell-derived mesodermal progenitors in a NOTCH-dependent manner.

Cell death & disease

Breuls N,Giarratana N,Yedigaryan L,Garrido GM,Carai P,Heymans S,Ranga A,Deroose C,Sampaolesi M

PA5-42902 was used in Western Blot to generate chemically defined human induced pluripotent stem cell-derived mesodermal progenitors (cdMiPs).

Mon Jul 05 00:00:00 EDT 2021

Valproic acid stimulates myogenesis in pluripotent stem cell-derived mesodermal progenitors in a NOTCH-dependent manner.

Cell death & disease

Breuls N,Giarratana N,Yedigaryan L,Garrido GM,Carai P,Heymans S,Ranga A,Deroose C,Sampaolesi M

PA5-42902 was used in Western Blot to generate chemically defined human induced pluripotent stem cell-derived mesodermal progenitors (cdMiPs).

Mon Jul 05 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Delta-like protein 1; Delta1; Drosophila Delta homolog 1; H-Delta-1; OTTHUMP00000017690

Gene Aliases: Delta; DELTA1; DL1; DLL1; UNQ146/PRO172

UniProt ID: (Human) O00548, (Rat) P97677

Entrez Gene ID: (Human) 28514, (Rat) 84010

cell fate determination somitogenesis somite specification heart looping Notch signaling pathway cell-cell signaling determination of left/right symmetry compartment pattern specification nervous system development auditory receptor cell fate commitment negative regulation of interleukin-10 production negative regulation of cell differentiation negative regulation of auditory receptor cell differentiation negative regulation of myeloid cell differentiation negative regulation of neuron differentiation positive regulation of Notch signaling pathway positive regulation of transcription from RNA polymerase II promoter inner ear development regulation of neurogenesis left/right axis specification proximal tubule development loop of Henle development neuronal stem cell population maintenance marginal zone B cell differentiation type B pancreatic cell development Notch receptor processing regulation of blood pressure positive regulation of cell proliferation negative regulation of cell proliferation proximal/distal pattern formation astrocyte development regulation of somitogenesis spinal cord development cerebellar molecular layer formation cerebellar Purkinje cell layer structural organization hemopoiesis cell differentiation regulation of cell adhesion negative regulation of epithelial cell differentiation negative regulation of glial cell apoptotic process organ growth regulation of growth negative regulation of epidermal cell differentiation negative regulation of myoblast differentiation positive regulation of endocytosis lateral inhibition skeletal muscle tissue growth regulation of skeletal muscle tissue growth positive regulation of skeletal muscle tissue growth neuron fate specification regulation of cell division retina development in camera-type eye retina morphogenesis in camera-type eye Notch signaling pathway involved in arterial endothelial cell fate commitment nephron development clathrin-mediated endocytosis endothelial tip cell fate specification skin epidermis development regulation of vascular endothelial growth factor signaling pathway positive regulation of sprouting angiogenesis regulation of energy homeostasis

Process(es)

Notch binding calcium ion binding protein binding Tat protein binding scaffold protein binding