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Invitrogen
DLL4 Polyclonal Antibody
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FIGURE: 1 / 5
DLL4 Antibody (PA5-46974) in ICC/IF
Immunocytochemistry analysis of DLL4 in immersion fixed bEnd.3 mouse endothelioma cell line. Samples were incubated in DLL4 polyclonal antibody (Product # PA5-46974) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-46974
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
C. elegans,
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DLL4 Ser28-Pro525
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, approximately 50% cross-reactivity with recombinant human DLL4 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Delta-like protein 4; Delta4; Drosophila Delta homolog 4; MGC126344
Gene Aliases: Delta4; Dll4
UniProt ID: (Mouse) Q9JI71
Entrez Gene ID: (Mouse) 54485
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
patterning of blood vessels
blood vessel remodeling
cardiac ventricle morphogenesis
cardiac atrium morphogenesis
ventricular trabecula myocardium morphogenesis
pericardium morphogenesis
cell communication
Notch signaling pathway
multicellular organism development
nervous system development
visual perception
negative regulation of cell proliferation
negative regulation of endothelial cell migration
positive regulation of gene expression
negative regulation of gene expression
cell differentiation
dorsal aorta morphogenesis
cellular response to vascular endothelial growth factor stimulus
negative regulation of blood vessel endothelial cell migration
cellular response to fibroblast growth factor stimulus
regulation of neurogenesis
response to stimulus
ventral spinal cord interneuron fate commitment
regulation of neural retina development
Notch signaling involved in heart development
blood vessel lumenization
negative regulation of cell migration involved in sprouting angiogenesis
negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
positive regulation of neural precursor cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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