Immunohistochemistry analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated human esophageal carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a DMAP1 polyclonal antibody (Product # PA1-886) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C-D(435) T I I D V V G A P L T R N S R(450) of mouse DMAP1.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
PA1-886 detects DNA methyltransferase 1 associated protein (DMAP1) from human and mouse cells.
PA1-886 has been successfully used in Western blot and Immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~52 kDa band which corresponds to DMAP1 from HeLa cell nuclear extracts.
PA1-886 immunizing peptide corresponds to amino acid residues 435-450 from mouse DMAP1. This sequence is completely conserved in human DMAP1. PA1-886 immunizing peptide (Cat. # PEP-118) is available for use in neutralization and control experiments.
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. DNA methyltransferase 1 associated protein (DMAP1) has been shown to interact directly with the N-terminus of DNA Methyltransferase 1 (Dnmt1) throughout DNA replication during S phase. DMAP1 has also been identified as a critical gene product in myotonic dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes.
PA1-886 was used in western blot to identify the YL1 protein as a part of the TRRAP/TIP60 HAT complex, and to study the roles of YL1 in chromatin modification and remodeling.
|Cai Y,Jin J,Florens L,Swanson SK,Kusch T,Li B,Workman JL,Washburn MP,Conaway RC,Conaway JW||The Journal of biological chemistry (280:13665)||2005|
Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans.
PA1-886 was used in western blot to investigate the evolutionary conservation of the NuA4 histone acetyltransferase complex.
|Doyon Y,Selleck W,Lane WS,Tan S,Côté J||Molecular and cellular biology (24:1884)||2004|
Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex.
PA1-886 was used in western blot to investigate the structure and function of the mammalian TRRAP/TIP60 histone acetyltransferase complex.
|Cai Y,Jin J,Tomomori-Sato C,Sato S,Sorokina I,Parmely TJ,Conaway RC,Conaway JW||The Journal of biological chemistry (278:42733)||2003|