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Invitrogen
DMAP1 Polyclonal Antibody
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FIGURE: 1 / 4
DMAP1 Antibody (PA1-886) in IHC (P)
Immunohistochemistry analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated human esophageal carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for ... View More
Product Details
PA1-886
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C-D(435) T I I D V V G A P L T R N S R(450) of mouse DMAP1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-886 detects DNA methyltransferase 1 associated protein (DMAP1) from human and mouse cells.
PA1-886 has been successfully used in Western blot and Immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~52 kDa band which corresponds to DMAP1 from HeLa cell nuclear extracts.
PA1-886 immunizing peptide corresponds to amino acid residues 435-450 from mouse DMAP1. This sequence is completely conserved in human DMAP1. PA1-886 immunizing peptide (Cat. # PEP-118) is available for use in neutralization and control experiments.
Target Information
DNA methyltransferase 1-associated protein 1 (DMAP1) is involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The protein also specifically potentiates DAXX-mediated repression of glucocorticoid receptor-dependent transcription. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. DMAP1 is found in a wide variety of living organisms from yeasts to humans. The human DMAP1 gene is localized on chromosome 1p34.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686L09142; DNA methyltransferase 1-associated protein 1; DNMAP1; DNMT1 associated protein 1; DNMT1 associated protein-1; DNMT1-associated protein 1; FLJ11543; MAT1-mediated transcriptional repressor; RP5-891H21.2; unnamed protein product
Gene Aliases: 1500016M21Rik; DMAP1; Dmtap1; DNMAP1; DNMTAP1; EAF2; KIAA1425; MEAF2; mKIAA1425; Mmtr; SWC4
UniProt ID: (Human) Q9NPF5, (Mouse) Q9JI44
Entrez Gene ID: (Human) 55929, (Mouse) 66233
negative regulation of transcription from RNA polymerase II promoter
DNA repair
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
positive regulation of protein import into nucleus
regulation of apoptotic process
response to ethanol
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
regulation of cell cycle
positive regulation of double-strand break repair via homologous recombination
regulation of double-strand break repair
transcription, DNA-templated
chromatin modification
regulation of growth
positive regulation of transcription factor import into nucleus
histone H4 acetylation
histone H2A acetylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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