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Invitrogen
DMAP1 Polyclonal Antibody
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FIGURE: 1 / 1
DMAP1 Antibody (PA5-96106) in WB
Western blot analysis of DMAP1 in extracts of various cell lines. Samples were incubated with DMAP1 Polyclonal antibody (Product # PA5-96106) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-96106
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human DMAP1 (NP_0619731)
Conjugate
Form
Liquid
Concentration
1.26 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MATGADVRDI LELGGPEGDA ASGTISKKDI INPDKKKSKK SSETLTFKRP EGMHREVYAL LYSDKKDAPP LLPSDTGQGY RTVKAKLGSK KVRPWKWMPF TNPARKDGAM FFHWRRAAEE GKDYPFARFN KTVQVPVYSE QEYQLYLHDD AWTKAETDHL FDLSRRFDLR FVVIHDRYDH QQFKKRSVED LKERYYHICA KLANVRAVPG TDLKIPVFDA GHERRRKEQL ERLYNRTPEQ VAEEEYLLQE LRKIEARKKE REKRSQDLQK LITAADTTAE QRRTERKAPK KKLPQKKEAE KPAVP; Positive Samples: SH-SY5Y, SW480, A375, HeLa, Jurkat, NIH/3T3, Mouse thymus, Rat thymus; Cellular Location: Cytoplasm, Nucleus
Target Information
DNA methyltransferase 1-associated protein 1 (DMAP1) is involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The protein also specifically potentiates DAXX-mediated repression of glucocorticoid receptor-dependent transcription. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. DMAP1 is found in a wide variety of living organisms from yeasts to humans. The human DMAP1 gene is localized on chromosome 1p34.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686L09142; DNA methyltransferase 1-associated protein 1; DNMAP1; DNMT1 associated protein 1; DNMT1 associated protein-1; DNMT1-associated protein 1; FLJ11543; MAT1-mediated transcriptional repressor; RP5-891H21.2; unnamed protein product
Gene Aliases: 1500016M21Rik; DMAP1; Dmtap1; DNMAP1; DNMTAP1; EAF2; KIAA1425; MEAF2; mKIAA1425; Mmtr; SWC4
UniProt ID: (Human) Q9NPF5, (Mouse) Q9JI44
Entrez Gene ID: (Human) 55929, (Mouse) 66233, (Rat) 298447
transcription corepressor activity
protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
RNA polymerase II repressing transcription factor binding
chromatin binding
molecular_function
methyltransferase activity
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
DNA repair
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
positive regulation of protein import into nucleus
regulation of apoptotic process
response to ethanol
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
regulation of cell cycle
positive regulation of double-strand break repair via homologous recombination
regulation of double-strand break repair
transcription, DNA-templated
chromatin modification
regulation of growth
positive regulation of transcription factor import into nucleus
histone H4 acetylation
histone H2A acetylation
methylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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