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Invitrogen
DMPK Polyclonal Antibody
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FIGURE: 1 / 4
DMPK Antibody (PA5-99271) in ICC/IF
Immunocytochemistry analysis of DMPK in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with DMPK polyclonal antibody (Product # PA5-99271) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-99271
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DMPK(Accession Q09013), corresponding to amino acid residues L20-E40.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total DMPK.
Target Information
DMPK is a serine/threonine kinase expressed in various muscle and nerve tissues. Mutations in the untranslated region of the DMPK gene are a characteristic of myotonic dystrophy. The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DM protein kinase; DM-kinase; DM1 protein kinase; DMK; DMPK; dystrophia myotonica-protein kinase; MT-PK; myotonic dystrophy associated protein kinase; Myotonic dystrophy protein kinase; myotonin protein kinase A; Myotonin-protein kinase; thymopoietin homolog
Gene Aliases: DM; DM1; DM1PK; DMK; DMPK; MDPK; MT-PK
UniProt ID: (Human) Q09013
Entrez Gene ID: (Human) 1760
regulation of sodium ion transport
protein phosphorylation
cellular calcium ion homeostasis
nuclear envelope organization
regulation of heart contraction
muscle cell apoptotic process
regulation of myotube differentiation
regulation of skeletal muscle contraction by calcium ion signaling
regulation of excitatory postsynaptic membrane potential involved in skeletal muscle contraction
peptidyl-serine phosphorylation
regulation of catalytic activity
regulation of synapse structural plasticity
regulation of cardiac conduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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