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Immunofluorescent analysis of DMRTA2 showing staining in the microtubules of U-251 cells using a DMRTA2 polyclonal antibody (Product # PA5-35234) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/ml, 1 h at 37°C). Nuclei were stained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human, Mouse, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine based on sequence homology.
DMRTA2 may be involved in sexual development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DMRT-like family A2; DMRT5; DMRTA2; Doublesex- and mab-3-related transcription factor 5; Doublesex- and mab-3-related transcription factor A2
CH73-349N16.1; DMRT5; DMRTA2