Invitrogen

DNAH9 Polyclonal Antibody

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DNAH9 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of mouse tracheal epithelial cells using an anti-Dynein Heavy chain polyclonal antibody (Product # PA5-45744). Primary Antibody Dilution:1:50; Secondary Antibody: Anti-rabbit alexa 488; Secondary Antibody Dilution: 1:1000.

DNAH9 Antibody in Immunohistochemistry (IHC) — Immunofluorescence analysis of mouse tracheal epithelial cells using an anti-Dynein Heavy chain polyclonal antibody (Product # PA5-45744). Primary Antibody Dilution:1:50; Secondary Antibody: Anti-rabbit alexa 488; Secondary Antibody Dilution: 1:1000.

DNAH9 Antibody in Western Blot (WB) — Western blot analysis of human placenta cells using an anti-Dynein Heavy chain polyclonal antibody (Product # PA5-45744).

Product Details

PA5-45744

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunohistochemistry (IHC)	1:50	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:50	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the C-terminal of human DNAH9 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2576916

Product Specific Information

Peptide sequence: DSQARDGAGA TREEKVKALL EEILERVTDE FNIPELMAKV EERTPYIVVA

Sequence homology: Cow: 83%; Dog: 100%; Guinea Pig: 83%; Horse: 92%; Human: 100%; Mouse: 92%; Rat: 92%

Target Information

This gene encodes the heavy chain subunit of axonemal dynein, a large multi-subunit molecular motor. Axonemal dynein attaches to microtubules and hydrolyzes ATP to mediate the movement of cilia and flagella. The gene expresses at least two transcript variants; additional variants have been described, but their full length nature has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genetic Defects in DNAH2 Underlie Male Infertility With Multiple Morphological Abnormalities of the Sperm Flagella in Humans and Mice.

Frontiers in cell and developmental biology

Hwang JY,Nawaz S,Choi J,Wang H,Hussain S,Nawaz M,Lopez-Giraldez F,Jeong K,Dong W,Oh JN,Bilguvar K,Mane S,Lee CK,Bystroff C,Lifton RP,Ahmad W,Chung JJ

Published figure using DNAH9 polyclonal antibody (Product # PA5-45744) in Immunohistochemistry

Tue Dec 21 00:00:00 EST 2021

Quantifying Ciliary Dynamics during Assembly Reveals Stepwise Waveform Maturation in Airway Cells.

American journal of respiratory cell and molecular biology

Oltean A,Schaffer AJ,Bayly PV,Brody SL

PA5-45744 was used in Immunocytochemistry-immunoflourescence to suggest that the stepwise variation in waveform development during ciliogenesis is dependent on cilia length and potentially on outer dynein arm assembly.

Mon Oct 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Axonemal beta dynein heavy chain 9; Ciliary dynein heavy chain 9; DNAH9 variant protein; Dynein axonemal heavy chain 9; dynein heavy chain 9, axonemal; dynein, axonemal, heavy polypeptide 9

Gene Aliases: 9030022M04; A230091C01; C230051G16; D11Ertd686e; DNAH17L; DNAH9; Dnahc9; DNEL1; DYH9; HL-20; HL20; KIAA0357; mKIAA0357

UniProt ID: (Human) Q9NYC9

Entrez Gene ID: (Human) 1770, (Mouse) 237806

Molecular Function: microtubule binding motor protein