Immunofluorescent analysis of DNAJB6 showing staining in the nucleus of NT2D1 cells. NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a DNAJB6 polyclonal antibody (Product # PA5-27577) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 156 and 326 of Human DNAJB6|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27577 targets DNAJB6 in WB applications and shows reactivity with Human samples.
The PA5-27577 immunogen is recombinant fragment corresponding to a region within amino acids 156 and 326 of Human DNAJB6.
This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.
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