Invitrogen

DNAJB6 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (19) DNAJB6 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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DNAJB6 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of DNAJB6 in human testis and pancreas tissues using DNAJB6 Polyclonal Antibody (Product # PA5-55031). Corresponding DNAJB6 RNA-seq data are presented for the same tissues.

DNAJB6 Antibody in Western Blot (WB) — Western blot analysis of DNAJB6 in control (vector only transfected HEK293T lysate) and DNAJB6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a DNAJB6 Polyclonal Antibody (Product # PA5-55031).

DNAJB6 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of DNAJB6 demonstrates antibody specificity. Immunohistochemical analysis of DNAJB6 using anti-DNAJB6 Polyclonal Antibody (Product # PA5-55031), shows significant staining of DNAJB6 in human testis and shows minimal or weak staining in human pancreas tissues. The relative expression levels of DNAJB6 within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human DNAJB6

Conjugate

Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2640685

Product Specific Information

Immunogen sequence: FDSPFEFGFT FRNPDDVFRE FFGGRDPFSF DFFEDPFEDF FGNRRGPRGS RSRGTGSFFS AFSGFPSFGS GFSSFDTGFT SFGSLGHGGL TSFSSTS

Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 94%.

Target Information

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DnaJ (Hsp40) homolog, subfamily B, member 6; DnaJ homolog subfamily B member 6; DnaJ-like 2 protein; Heat shock protein J2; HHDJ1; HSJ-2; MRJ; MSJ-1

Gene Aliases: DJ4; DnaJ; DNAJB6; HHDJ1; HSJ-2; HSJ2; LGMD1D; LGMD1E; MRJ; MSJ-1; MSJ1

UniProt ID: (Human) O75190

Entrez Gene ID: (Human) 10049

Function(s)