Invitrogen

DNAJB6 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (17) DNAJB6 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

DNAJB6 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of DNAJB6 in human testis and pancreas tissues using DNAJB6 Polyclonal Antibody (Product # PA5-55031). Corresponding DNAJB6 RNA-seq data are presented for the same tissues.

DNAJB6 Antibody in Western Blot (WB) — Western blot analysis of DNAJB6 in control (vector only transfected HEK293T lysate) and DNAJB6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a DNAJB6 Polyclonal Antibody (Product # PA5-55031).

DNAJB6 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of DNAJB6 demonstrates antibody specificity. Immunohistochemical analysis of DNAJB6 using anti-DNAJB6 Polyclonal Antibody (Product # PA5-55031), shows significant staining of DNAJB6 in human testis and shows minimal or weak staining in human pancreas tissues. The relative expression levels of DNAJB6 within each tissue is shown using RNA-Seq. {RE}

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human DNAJB6

Conjugate

Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2640685

Product Specific Information

Immunogen sequence: FDSPFEFGFT FRNPDDVFRE FFGGRDPFSF DFFEDPFEDF FGNRRGPRGS RSRGTGSFFS AFSGFPSFGS GFSSFDTGFT SFGSLGHGGL TSFSSTS

Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 94%.

Target Information

In prokaryotes, the heat shock response is characterized by the induction of several proteins including DnaJ, DnaK, GroEL and GroES. DnaK is a member of the HSP70 family, and like other members, exhibits ATPase activity which facilitates the release of bound proteins and disaggregation of protein complexes within the cell. Members of this protein group serve as molecular chaperones. Levels of DnaJ and DnaK are closely linked and, in E. coli, are constituents of a single operon. DnaJ regulates DnaK in several different systems. Relative to DnaK, the level of DnaJ synthesis is quite low.

Studies have shown that DnaJ and DnaK, together with GrpE, cooperatively interact with firefly luciferase as it unfolds during thermal denaturation allowing renaturation of the protein upon return to normal temperatures. Also, DnaK together with DnaJ stimulates the binding of RepA initiator protein to the origin of P1 phage in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DJ4; DnaJ; DnaJ (Hsp40) homolog, subfamily B, member 6; DnaJ homolog subfamily B member 6; DnaJ-like 2 protein; Heat shock protein J2; HHDJ1; HSJ-2; HSJ2; MRJ; MSJ-1

Gene Aliases: DJ4; DnaJ; DNAJB6; HHDJ1; HSJ-2; HSJ2; LGMD1D; LGMD1E; MRJ; MSJ-1; MSJ1

UniProt ID: (Human) O75190

Entrez Gene ID: (Human) 10049

Function(s)