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Invitrogen
DNAJC10 Polyclonal Antibody
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FIGURE: 1 / 2
DNAJC10 Antibody (PA5-143983) in Flow
Flow cytometry analysis of DNAJC10 in HELA cells using DNAJC10 Polyclonal Antibody (Product # PA5-143983), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control a... View More
Product Details
PA5-143983
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human DNAJC10 recombinant protein (Position: D33-L793).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075197
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HELA whole cell, human HEPG2 whole cell, human U87 whole cell, rat stomach tissue, rat RH35 whole cell, mouse stomach tissue, mouse HEPA1-6 whole cell. IHC: human breast cancer tissue, human placenta tissue, human prostate cancer tissue, human gastric cancer tissue. ICC/IF: A431 cell. Flow: HELA cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This endoplasmic reticulum co-chaperone may play a role in protein folding and translocation across the endoplasmic reticulum membrane. DNAJC10 may act as a co-chaperone for HSPA5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily C, member 10; DnaJ homolog subfamily C member 10; Endoplasmic reticulum DNA J domain-containing protein 5; Endoplasmic reticulum DnaJ-PDI fusion protein 1; ER-resident protein ERdj5; J domain-containing PDI-like protein; J domain-containing protein disulfide isomerase-like protein; J-domain-containing protein disulfide isomerase-like protein; Macrothioredoxin; MTHr; protein disulfide isomerase family A, member 19
Gene Aliases: 1200006L06Rik; D2Ertd706e; DNAJC10; ERDJ5; JPDI; MTHr; PDIA19; UNQ495/PRO1012
UniProt ID: (Human) Q8IXB1, (Rat) Q498R3, (Mouse) Q9DC23
Entrez Gene ID: (Human) 54431, (Rat) 295690, (Mouse) 66861
ATPase activator activity
protein binding
protein disulfide oxidoreductase activity
disulfide oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor
Hsp70 protein binding
chaperone binding
ATPase binding
misfolded protein binding
peptide-methionine (S)-S-oxide reductase activity
oxidoreductase activity
peptide-methionine (R)-S-oxide reductase activity
methionine-R-sulfoxide reductase activity
chaperone
negative regulation of protein phosphorylation
ER-associated ubiquitin-dependent protein catabolic process
positive regulation of ATPase activity
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
cell redox homeostasis
oxidation-reduction process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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