Immunofluorescent analysis of DNMT1 (red) in human melanocytes. Cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.2% Triton X-100 in PBS for 10 minutes at room temperature, and blocked with 5% normal goat serum for 1 hour at room temperature. Cells were probed with a DNMT1 polyclonal antibody (Product # PA5-30581) at a dilution of 1:500 for 1 hour at room temperature, washed with PBS, and incubated with an anti-rabbit Alexa-Fluor 555-conjugated secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a fluorescent microscope at 20X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 82 and 178 of Human DNMT1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30581 targets DNMT1 in IF, IHC (P), ChIP and WB applications and shows reactivity with Human samples.
The PA5-30581 immunogen is synthetic peptide corresponding to a region within amino acids 82 and 178 of Human DNMT1.
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.