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Invitrogen
DNMT3B Polyclonal Antibody
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FIGURE: 1 / 7
DNMT3B Antibody (PA5-85549) in ICC/IF
DNMT3B Polyclonal Antibody detects DNMT3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3B protein stained by DNMT3B Polyclonal Antibody (Product # PA5-85549) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 µm.
Product Details
PA5-85549
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3B. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.29 mg/mL
Amount
129 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Pig (85%).
Positive Control: HeLa, HeLa nuclear, GFP-tagged DNMT3B-transfested 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: de novo DNA methyltransferase; DNA (cytosine-5)-methyltransferase 3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA cytosine-5--methyltransferase 3 beta; DNA methyltransferase 3B; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; Dnmt3; Dnmt3b; DNMT3B1; DNMT3B4; DNMT3B5; DNMT3B7; m.MmuIIIB; MGC1090; unnamed protein product
Gene Aliases: DNMT3B; FSHD4; ICF; ICF1; M.HsaIIIB
UniProt ID: (Human) Q9UBC3
Entrez Gene ID: (Human) 1789, (Rat) 444985
DNA binding
transcription corepressor activity
DNA (cytosine-5-)-methyltransferase activity
protein binding
methyltransferase activity
zinc ion binding
DNA-methyltransferase activity
transferase activity
metal ion binding
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
chromatin binding
histone deacetylase binding
unmethylated CpG binding
DNA methyltransferase
negative regulation of transcription from RNA polymerase II promoter
regulation of gene expression
positive regulation of gene expression
methylation
response to hypoxia
DNA methylation
methylation-dependent chromatin silencing
regulation of gene expression by genetic imprinting
response to toxic substance
response to ionizing radiation
DNA methylation on cytosine within a CG sequence
response to activity
response to caffeine
protein complex localization
response to estradiol
response to vitamin A
response to cocaine
response to drug
positive regulation of neuron differentiation
negative regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
positive regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
inactivation of X chromosome by DNA methylation
cellular response to amino acid stimulus
cellular response to hyperoxia
cellular response to dexamethasone stimulus
C-5 methylation of cytosine
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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