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Invitrogen
DOCK4 Polyclonal Antibody
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FIGURE: 1 / 3
DOCK4 Antibody (PA5-143947) in IHC (P)
Immunohistochemistry analysis of DOCK4 in paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with DOCK4 Polyclonal antibody (Product # PA5-143947) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-rab... View More
Product Details
PA5-143947
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human DOCK4 recombinant protein (Position: K191-I1837).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, rat L6 whole cell. IHC: human skeletal muscle tissue. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The DOCK4 gene encodes the dedicator of cytokinesis 4 protein, a member of the DOCK family, which functions as a guanine nucleotide exchange factor (GEF) for small Rho GTPases, specifically Rac1 and Rap1. DOCK4 is critical for regulating various cellular processes, including cell migration, polarity, and cytoskeletal organization. It plays a significant role in maintaining the integrity of endothelial barriers and mediating intercellular junctions. Mutations or altered expression of DOCK4 have been implicated in cancer progression, particularly in tumor metastasis and invasion, by affecting the adhesion and migration of cancer cells. Additionally, DOCK4 is involved in neurodevelopmental processes and has been associated with psychiatric disorders, reflecting its importance in brain function and development. Understanding the roles of DOCK4 in both health and disease could provide insights into therapeutic strategies for cancer and neurological disorders where these pathways are disrupted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activator of GTPase signaling; dedicator of cytokinesis 4; Dedicator of cytokinesis protein 4; unnamed protein product
Gene Aliases: DOCK4; KIAA0716; RGD1561724
UniProt ID: (Human) Q8N1I0
Entrez Gene ID: (Human) 9732, (Rat) 366608
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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