Proteintech

DOCK9 Polyclonal Antibody

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DOCK9 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 18987-1-AP (DOCK9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DOCK9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 18987-1-AP ( DOCK9 Antibody) at dilution of 1:200 (under 40x lens).

DOCK9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 18987-1-AP ( DOCK9 Antibody) at dilution of 1:200 (under 10x lens).

DOCK9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens).

DOCK9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens).

DOCK9 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

DOCK9 Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

DOCK9 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DOCK9 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

DOCK9 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DOCK9 Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DOCK9 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DOCK9 Antibody in Immunoprecipitation (IP) — IP result of anti-DOCK9 (IP:18987-1-AP, 4ug; Detection:18987-1-AP 1:800) with mouse brain tissue lysate 4000ug.

Product Details

18987-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Target Information

DOCK9 is a guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression of DOCK9 induces filopodia formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cdc42 guanine nucleotide exchange factor zizimin-1; Dedicator of cytokinesis protein 9; Zizimin-1

Gene Aliases: AA959601; AW538057; B230309H04Rik; D14Wsu89e; DOCK9; KIAA1058; mKIAA1058; ZIZ1; ZIZIMIN1

UniProt ID: (Human) Q9BZ29

Entrez Gene ID: (Human) 23348, (Mouse) 105445

Molecular Function: guanyl-nucleotide exchange factor

small GTPase mediated signal transduction blood coagulation biological_process positive regulation of GTPase activity cell-cell adhesion

Process(es)

guanyl-nucleotide exchange factor activity protein binding cadherin binding involved in cell-cell adhesion Rho guanyl-nucleotide exchange factor activity Rho GTPase binding