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Invitrogen
DPF2 Polyclonal Antibody
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FIGURE: 1 / 6
DPF2 Antibody (PA5-27651) in ICC/IF
Immunofluorescence analysis of Zinc finger protein ubi-d4 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with DPF2 Polyclonal Antibody (Product # PA5-27651) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ... View More
Product Details
PA5-27651
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human DPF2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.67 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: DPF2 tansfected 293T cell.
Predicted reactivity: Mouse (98%), Rat (99%), Xenopus laevis (84%), Chicken (90%), Chimpanzee (100%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptosis response zinc finger protein; BAF45D; BRG1-associated factor 45D; D4, zinc and double PHD fingers family 2; neuro-d4/requiem; Protein requiem; requiem; requiem, apoptosis response zinc finger; Zinc finger protein ubi-d4
Gene Aliases: 2210010M07Rik; BAF45D; DPF2; REQ; ubi-d4; UBID4
UniProt ID: (Human) Q92785, (Mouse) Q61103
Entrez Gene ID: (Human) 5977, (Mouse) 19708
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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