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          • Primary Antibodies ›
          • DPH2 Antibodies

          Invitrogen

          DPH2 Polyclonal Antibody

          View all (15) DPH2 antibodies

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          Cite DPH2 Polyclonal Antibody

          Additional Information:

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          • Antibody Testing Data (2)
          DPH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          DPH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          DPH2 Antibody (PA5-30008) in IHC (P)

          Immunohistochemical analysis of paraffin-embedded human breast cancer, using DPH2 (Product # PA5-30008) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          DPH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DPH2 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          PA5-30008

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human DPH2. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2547482

          Product Specific Information

          Recommended positive controls: HeLa.

          Predicted reactivity: Mouse (87%), Rat (87%), Bovine (93%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: diphthamide biosynthesis protein 2 homolog-like 2; diphthamide biosynthesis-like protein 2; diphtheria toxin resistance protein 2; diptheria toxin resistance protein required for diphthamide biosynthesis-like 2; DPH2 homolog; DPH2-like 2; S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 2; unnamed protein product

          View more View less

          Gene Aliases: DEDSSH2; DPH2L2

          View more View less

          UniProt ID: (Human) Q9BQC3

          View more View less

          Entrez Gene ID: (Human) 1802

          View more View less

          Function(s)
          protein binding 4 iron, 4 sulfur cluster binding 2-(3-amino-3-carboxypropyl)histidine synthase activity
          Process(es)
          peptidyl-diphthamide biosynthetic process from peptidyl-histidine
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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