Immunofluorescent analysis of paraffin-embedded human brain tissue using a DPT polyclonal antibody (Product # PA5-14396) at a dilution of 0.05 mg/ml, followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 102-128 amino acids from the central region of human DPT|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, mouse and porcine based on sequence homology.
DPT is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. This protein is found in various tissues and many of its tyrosine residues are sulphated. The protein is postulated to modify the behavior of TGF-beta through interaction with decorin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.