|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 50-100 of human DRAM.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is expected to react with canine, monkey and opossum samples based on sequence homology.
DRAM is an important lysosomal membrane protein of the p53 tumor suppressor pathway. It is required for the induction of macroautophagy and as an effector of p53-mediated death. DRAM expression is induced due to cellular stress via endogenous p53 leading to bulk degradation of cytoplasmic components. It is necessary but not sufficient for apoptotic cell death from p53, while over expression of DRAM alone causes minimal cell death. Quantitative PCR revealed that DRAM was significantly down regulated in tumor cell lines by methylation-dependent transcriptional silencing, particularly in cancers lacking other mechanisms of p53 inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.