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Agrisera
DRB4/Double-stranded RNA-binding protein 4 Polyclonal Antibody
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FIGURE: 1 / 2
DRB4/Double-stranded RNA-binding protein 4 Antibody (AS15-3104) in WB
100 µg of total protein from Arabidopsis thaliana, drb 4, and 35 S-DRB 4 leaves extracted with isolation buffer (50 m M Tris-HCl, p H 7.5,10% glycerol, 150 m M Na Cl, 10 m M Mg Cl 2,5 m M EDTA, 5 m M DTT, and 1 X protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and denatured by boiling at 100°C. The proteins were separated on 10% SDS-PAGE and blotted 1.5 hrs to PVDF membrane using wet transfer. Bl... View More
Product Details
AS15-3104
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana DRB4 sequence.
Conjugate
Form
Lyophilized
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
DRB4 is a protein-coding gene within the major histocompatibility complex class II region and encodes an HLA-DR beta chain that pairs with the invariant HLA-DRA alpha chain to form a cell-surface, membrane-anchored heterodimer on professional antigen-presenting cells. The encoded beta chain is synthesized as a type I membrane glycoprotein precursor (approximately 26-28 kDa) with an N-terminal signal peptide, two extracellular domains that create the peptide-binding platform and Ig-like domain, a single-pass transmembrane helix, and a short cytoplasmic tail; gene organization classically places the leader sequence in exon 1, the extracellular domains in exons 2-3, the transmembrane segment in exon 4, and the cytoplasmic tail in exon 5 (with additional untranslated or alternatively annotated exons reported on alternate loci). Functionally, the DRB4-containing HLA-DR complex binds peptides generated mainly in the endocytic/lysosomal pathway from extracellular or vesicular proteins and displays them at the cell surface for surveillance by CD4 T cells, thereby shaping adaptive immune responses; extensive allelic polymorphism in the beta chain is a key determinant of peptide-binding specificity and underlies its importance in histocompatibility matching and disease association studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATTIF3K1; double-stranded-RNA-binding protein 4
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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