Invitrogen

DRD1 Polyclonal Antibody

View all (34) DRD1 antibodies

Questions & Answers

Tech Support

Questions & Answers

Tech Support

Antibody Testing Data (3)

DRD1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

DRD1 Antibody (PA5-85084) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of DRD1 was performed in DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: DRD1 Polyclonal Antibody (Product # PA5-85084) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Product Image Gallery

DRD1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of DRD1 was performed in DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: DRD1 Polyclonal Antibody (Product # PA5-85084) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

DRD1 Antibody in Western Blot (WB) — Western blot analysis of DRD1 in A) IMR32 cells using DRD1 polyclonal antibody (Product # PA5-85084) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS PAGE.

DRD1 Antibody in Western Blot (WB) — Western Blot using DRD1 Polyclonal Antibody (Product # PA5-85084). Sample (30 µg of whole cell lysate). Lane A: IMR32 10% SDS PAGE. DRD1 Polyclonal Antibody (Product # PA5-85084) diluted at 1:1,000.

Product Details

PA5-85084

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Dopamine Receptor D1.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2792233

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Pig (100%), Rhesus Monkey (100%).

Positive Control: NT2D1, IMR32.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: D(1A) dopamine receptor; D1a receptor; Dopamine 1 receptor; Dopamine D1 receptor; dopamine receptor D1A; Dopamine-1A receptor

Gene Aliases: D1a; DADR; Drd-1; DRD1; DRD1A

UniProt ID: (Human) P21728

Entrez Gene ID: (Human) 1812, (Rat) 24316

Molecular Function: G-protein coupled receptor

temperature homeostasis conditioned taste aversion behavioral fear response synaptic transmission, dopaminergic response to amphetamine negative regulation of protein kinase activity protein import into nucleus G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger adenylate cyclase-activating G-protein coupled receptor signaling pathway activation of adenylate cyclase activity adenylate cyclase-activating dopamine receptor signaling pathway synapse assembly learning memory mating behavior grooming behavior adult walking behavior visual learning positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway astrocyte development response to activity dopamine transport transmission of nerve impulse neuronal action potential regulation of vasoconstriction calcium-mediated signaling dentate gyrus development striatum development orbitofrontal cortex development cerebral cortex GABAergic interneuron migration positive regulation of cell migration negative regulation of cell migration peristalsis positive regulation of cAMP biosynthetic process response to food response to estradiol response to retinoic acid cellular response to insulin stimulus response to nicotine operant conditioning social behavior response to antidepressant regulation of dopamine metabolic process vasodilation negative regulation of circadian sleep/wake cycle, sleep dopamine metabolic process response to drug maternal behavior response to amino acid positive regulation of potassium ion transport response to morphine response to ethanol positive regulation of membrane potential glucose import habituation sensitization behavioral response to cocaine regulation of long-term neuronal synaptic plasticity response to steroid hormone positive regulation of release of sequestered calcium ion into cytosol positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway regulation of dopamine uptake involved in synaptic transmission positive regulation of synaptic transmission, glutamatergic prepulse inhibition phospholipase C-activating dopamine receptor signaling pathway long-term synaptic potentiation long term synaptic depression cellular response to hypoxia cellular response to catecholamine stimulus positive regulation of long-term synaptic potentiation positive regulation of feeding behavior startle response intracellular protein transport muscle contraction G-protein coupled receptor signaling pathway dopamine receptor signaling pathway chemical synaptic transmission locomotory behavior feeding behavior associative learning response to organonitrogen compound response to organic cyclic compound hippocampus development phosphatidylinositol catabolic process response to cocaine regulation of ion transport positive regulation of adenylate cyclase activity phosphatidylinositol metabolic process

Process(es)

dopamine neurotransmitter receptor activity, coupled via Gs G-protein alpha-subunit binding dopamine neurotransmitter receptor activity protein binding drug binding protein phosphatase binding angiotensin receptor binding D3 dopamine receptor binding protein complex binding dopamine binding protein heterodimerization activity ATPase binding G-protein coupled receptor activity receptor binding