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Product Details
PA5-55750
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human DTL. Recombinant protein control fragment (Product #RP-94290).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2640810
Product Specific Information
Immunogen sequence: TVVLFQDENT LVSAGAVDGI IKVWDLRKNY TAYRQEPIAS KSFLYPGSST RKLGYSSLIL DSTGSTLFAN CTDDNIYMFN MTGLKTSPVA IF
Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 95%.
Target Information
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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