Invitrogen

DUS3L Polyclonal Antibody

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DUS3L Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of DUS3L in human cerebral cortex tissue shows strong cytoplasmic positivity in neuronal cells. Samples were probed using a DUS3L Polyclonal Antibody (Product # PA5-59757).

DUS3L Antibody in Western Blot (WB) — Western blot analysis of DUS3L in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DUS3L Polyclonal Antibody (Product # PA5-59757).

DUS3L Antibody in Immunoprecipitation (IP) — Immunoprecipitation of DUS3L was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal DUS3L antibody (Product # PA5-59757) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.

DUS3L Antibody in RNA Immunoprecipitation (RIP) — RNA immunoprecipitation (RIP) western of DUS3L was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal DUS3L antibody (Product # PA5-59757) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.

Product Details

PA5-59757

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-
Immunoprecipitation (IP)	Assay-dependent	-
RNA Immunoprecipitation (RIP)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human DUS3L View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2640828

Product Specific Information

Immunogen sequence: TVEVDFVDIN VGCPIDLVYK KGGGCALMNR STKFQQIVRG MNQVLDVPLT VKIRTGVQER VNLAHRLLPE LRDWGVALVT LHGRSREQRY TKLADWQ

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 91%.

Target Information

DUS3L gene ontology annotations related to this gene include flavin adenine dinucleotide binding; metal ion binding; tRNA dihydrouridine synthase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: dihydrouridine synthase 3-like; putative zinc finger protein MA23; tRNA-dihydrouridine synthase 3-like; tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like

Gene Aliases: AI662135; AW557805; DUS3; DUS3L

UniProt ID: (Human) Q96G46, (Mouse) Q91XI1, (Rat) Q3KRC5

Entrez Gene ID: (Human) 56931, (Mouse) 224907, (Rat) 301122

Molecular Function: RNA metabolism protein RNA processing factor nucleic acid metabolism protein