Invitrogen

DUS3L Polyclonal Antibody

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DUS3L Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of DUS3L in human cerebral cortex tissue shows strong cytoplasmic positivity in neuronal cells. Samples were probed using a DUS3L Polyclonal Antibody (Product # PA5-59757).

DUS3L Antibody in Western Blot (WB) — Western blot analysis of DUS3L in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DUS3L Polyclonal Antibody (Product # PA5-59757).

DUS3L Antibody in Immunoprecipitation (IP) — Immunoprecipitation of DUS3L was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal DUS3L antibody (Product # PA5-59757) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:200-1:500

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human DUS3L

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2640828

Product Specific Information

Immunogen sequence: TVEVDFVDIN VGCPIDLVYK KGGGCALMNR STKFQQIVRG MNQVLDVPLT VKIRTGVQER VNLAHRLLPE LRDWGVALVT LHGRSREQRY TKLADWQ

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 91%.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: dihydrouridine synthase 3-like; DUS3; putative zinc finger protein MA23; tRNA-dihydrouridine synthase 3-like; tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like

Gene Aliases: AI662135; AW557805; DUS3; DUS3L

UniProt ID: (Human) Q96G46, (Mouse) Q91XI1, (Rat) Q3KRC5

Entrez Gene ID: (Human) 56931, (Mouse) 224907, (Rat) 301122

Function(s)

tRNA dihydrouridine synthesis oxidation-reduction process tRNA processing

Process(es)