Invitrogen

DUSP10 Polyclonal Antibody

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DUSP10 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DUSP10 in NIH-3T3 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with DUSP10 polyclonal antibody (Product # PA5-106794) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

DUSP10 Antibody in Western Blot (WB) — Western blot analysis of DUSP10 in NIH-3T3 cell lysate (left lane: treated with blocking peptide). Samples were incubated with DUSP10 polyclonal antibody (Product # PA5-106794).

Product Details

PA5-106794

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human DUSP10(Accession Q9Y6W6), corresponding to amino acid residues N369-E392. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2854462

Target Information

Microsomal long and very long chain fatty acid elongation uses malonyl-CoA as the 2-carbon donor and consists of 4 sequential reactions. GPSN2 catalyzes the final step, reducing trans-2, 3-enoyl-CoA to saturated acyl-CoA. Microsomal long and very long chain fatty acid elongation uses malonyl-CoA as the 2-carbon donor and consists of 4 sequential reactions. TER catalyzes the final step, reducing trans-2, 3-enoyl-CoA to saturated acyl-CoA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Knockdown of TRIM9 attenuates irinotecan‑induced intestinal mucositis in IEC‑6 cells by regulating DUSP6 expression via the P38 pathway.

Molecular medicine reports

Zhao W,Wang Q

PA5-106794 was used in Western Blotting to investigate the effect of Tripartite motif family 9 on irinotecan‚induced intestinal mucositis in the rat intestinal epithelial cell line IEC‚6.

Wed Dec 01 00:00:00 EST 2021

Knockdown of TRIM9 attenuates irinotecan‑induced intestinal mucositis in IEC‑6 cells by regulating DUSP6 expression via the P38 pathway.

Molecular medicine reports

Zhao W,Wang Q

PA5-106794 was used in Western Blotting to investigate the effect of Tripartite motif family 9 on irinotecan‚induced intestinal mucositis in the rat intestinal epithelial cell line IEC‚6.

Wed Dec 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: dual specificity phosphatase MKP-5; Dual specificity protein phosphatase 10; MAP kinase phosphatase 5; Mitogen-activated protein kinase phosphatase 5; serine/threonine specific protein phosphatase

Gene Aliases: 2610306G15Rik; AI158871; DUSP10; MKP-5; MKP5

UniProt ID: (Human) Q9Y6W6, (Mouse) Q9ESS0

Entrez Gene ID: (Human) 11221, (Mouse) 63953, (Rat) 63995

Molecular Function: protein phosphatase