|Tested species reactivity||Dog, Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to human Dual specificity phosphatase 3.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27774 detects DUSP3 from human, mouse, rat, and canine samples.
PA1-27774 may be successfully used in Western blot applications. By Western blot, this antibody has only been tested on fusion protein.
The PA1-27510 immunogen is a synthetic peptide corresponding to human Dual specificity phosphatase 3.
This protein shows activity both toward tyrosine-protein phosphate as well as with serine-protein phosphate. The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK. SAPK/JNK. p38). which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases. different tissue distribution and subcellular localization. and different modes of inducibility of their expression by extracellular stimuli. This protein maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues. mutation screening in breast cancer pedigrees and in sporadic tumors was negative. leading to the conclusion that this gene is not BRCA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.