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Invitrogen
DUSP3 Polyclonal Antibody
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FIGURE: 1 / 1
DUSP3 Antibody (PA5-79174) in WB
Western blot analysis of DUSP3 using DUSP3 Polyclonal Antibody (Product # PA5-79174). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. Lane 2: human MDA-MB-453 whole cell lysates. Lane 3: human Caco-2 whole cell lysates. Lane 4: human HepG2 whole cell lysates. Lane 5: rat skeletal muscle tissue lysates. Lane 6: rat heart tissue lysates. Lane 7: mouse sk... View More
Product Details
PA5-79174
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DUSP3 (155-171aa RQNREIGPNDGFLAQLC).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human MDA-MB-453 whole cell, human Caco-2 whole cell, human HepG2 whole cell, rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue.
Target Information
Protein tyrosine phosphatases (PTPs) are a group of enzymes that remove phosphate groups from phosphorylated tyrosine residues on proteins. Together with tyrosine kinases, PTPs regulate the phosphorylation state of many important signaling molecules, such as the MAP kinase family. Recently, increasing attention has been focused on the growing family of PTPs. Like PTKs, PTPs have been implicated in cell signaling, cell growth and proliferation, and oncogenic transformation. Moreover, some PTPs can be involved in cell cycle regulation and embryogenesis. Dual specificity phosphatases (DSPs) are an emerging subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which appears to be selective for dephosphorylating the critical phosphothreonine and phosphotyrosine residues. The prototypical DSP is the VH1 gene in vaccinia virus expressed in late-stage viral infection. A shallow active site pocket in VHR allows for the hydrolysis of phosphorylated serine, threonine, or tyrosine protein residues, whereas the deeper active site of protein tyrosine phosphatases (PTPs) restricts substrate specificity to only phosphotyrosine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related)-like; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUSP 3; DUSP-3; serine/threonine specific protein phosphatase; T-DSP11; Vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; VHR
Gene Aliases: 2210015O03Rik; 5031436O03Rik; DUSP3; RGD1560049; T-DSP11; VHR
UniProt ID: (Human) P51452, (Mouse) Q9D7X3
Entrez Gene ID: (Human) 1845, (Mouse) 72349, (Rat) 498003
phosphoprotein phosphatase activity
protein serine/threonine phosphatase activity
protein tyrosine phosphatase activity
protein binding
cytoskeletal protein binding
protein tyrosine/serine/threonine phosphatase activity
hydrolase activity
phosphatase activity
protein kinase binding
receptor signaling protein tyrosine kinase inhibitor activity
receptor tyrosine kinase binding
MAP kinase phosphatase activity
protein tyrosine kinase binding
molecular_function
inactivation of MAPK activity
in utero embryonic development
protein dephosphorylation
dephosphorylation
peptidyl-tyrosine dephosphorylation
negative regulation of MAPK cascade
positive regulation of mitotic cell cycle
negative regulation of JNK cascade
negative regulation of T cell receptor signaling pathway
negative regulation of T cell activation
negative regulation of ERK1 and ERK2 cascade
negative regulation of signal transduction
negative regulation of cell migration
negative regulation of epidermal growth factor receptor signaling pathway
negative regulation of chemotaxis
regulation of focal adhesion assembly
cellular response to epidermal growth factor stimulus
positive regulation of focal adhesion disassembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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