Western blot staining of HEK293 overexpressing Human DYDC1 lysate (Myc tagged and loaded in Labe A and C) stained using PA5-19251 at a concentration of 1 µg/ml (lane A). Lane C was stained with anti-MYC Tag at a dilution of 1/1000. Lane B was loaded with mock-transfected HEK293 lysate and was stained with PA5-19251 at a concentration of 0.1µg/ml. Primary antibody incubation was performed for 1 hour and detection was performed by chemiluminescence.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (TLAEISDRYGAPN) corresponding to the internal amino acids of DYDC1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.