Invitrogen

DYRK1A Polyclonal Antibody

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Protocols

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DYRK1A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of DYRK1A in U20S cell. Antigen retrieval was performed on the tissue using IHC enzyme antigen retrieval reagent and blocked with 10% goat serum. Samples were incubated with DYRK1A polyclonal antibody (Product # PA5-95499) at a 2 µg/mL dilution, followed by Cy3 conjugated Goat anti-rabbit IgG using a 1:100 dilution and DAPI.

DYRK1A Antibody in Western Blot (WB) — Western blot was performed using Anti-DYRK1A Polyclonal Antibody (Product # PA5-95499) and a 90kDa band corresponding to Dual specificity tyrosine-phosphorylation-regulated kinase 1A was observed across all the tested cell lines and tissues, except HEK-293 and Mouse Brain. Nuclear enriched extracts (40 µg lysate) of HEK-293 (Lane 1), K-562 (Lane 2), HEL 92.1.7 (Lane 3), SH-SY5Y (Lane 4), PC-3 (Lane 5), HeLa (Lane 6), Jurkat (Lane 7), Mouse Ovary (Lane 8), Mouse Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.3 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Product Details

PA5-95499

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunocytochemistry (ICC/IF)	2ug/mL	-
Flow Cytometry (Flow)	1-3ug/mL/1x10^6 cells	-
ELISA (ELISA)	0.1-0.5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E.coli-derived human DYRK1A recombinant protein (Position: H38-M269). View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 4mg trehalose
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2807301

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Dual Specificity Yak1-related kinase; hMNB; HP86; mnb protein kinase homolog hp86; MNB/DYRK protein kinase; MNBH; MP86; OTTHUMP00000109090; Protein kinase minibrain homolog; RP86; serine/threonine kinase MNB; serine/threonine-specific protein kinase

Gene Aliases: 2310043O08Rik; D16Ertd272e; D16Ertd493e; DYRK; DYRK1; DYRK1A; ENSMUSG00000074897; Gm10783; HP86; mmb; MNB; MNBH; Mp86; MRD7; PSK47

UniProt ID: (Human) Q13627, (Mouse) Q61214, (Rat) Q63470

Entrez Gene ID: (Human) 1859, (Mouse) 13548, (Rat) 25255

Function(s)

regulation of alternative mRNA splicing, via spliceosome protein phosphorylation circadian rhythm phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation peptidyl-tyrosine phosphorylation negative regulation of DNA damage response, signal transduction by p53 class mediator protein autophosphorylation negative regulation of mRNA splicing, via spliceosome positive regulation of protein deacetylation nervous system development regulation of endocytosis

Process(es)

protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding identical protein binding protein self-association tau protein binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

DYRK1A Polyclonal Antibody

Product Details

PA5-95499

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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