Invitrogen

Dectin 1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (24) Dectin 1 (Clec7a) antibodies

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Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Dectin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of CLEC7A in Jurkat cells with Dectin 1 Polyclonal Antibody (Product # PA5-34382) at 20 µg/mL.

Dectin 1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry of CLEC7A in human spleen tissue with Dectin 1 Polyclonal Antibody (Product # PA5-34382) at 5 µg/mL.

Dectin 1 Antibody in Western Blot (WB) — Western Blot analysis of CLEC7A in rat spleen tissue lysate with Dectin 1 Polyclonal Antibody (Product # PA5-34382) at (A) 1 and (B) 2 µg/mL.

Dectin 1 Antibody — Loss of laminarin activity in Dectin-1 knockdown cells A. Dectin-1 in RAW or THP-1 cells was silenced using lentiviral particles containing shRNA targeted against the murine and human Clec7a gene. Control cells were also lentivirally transduced with a non-targeting shRNA against GFP. After antibiotic selection, single cell clones were propagated and screened for loss of Dectin-1 mRNA expression using qPCR (left) or protein expression (right). For protein analysis in RAWs, cells were pretreated with 30 µg/ml zymosan. Numbers under blots represent relative level of Dectin-1 protein vs. control cells as determined by quantification using the software ImageJ and normalization to actin protein levels. Dashed lines indicate image splicing to remove irrelevant lanes. B. TNFa production in response to 24 h treatment of the indicated cells, control (red) or Dectin-1 knockdown (black; clone 6 and clone 3 for RAW and THP-1, respectively), with increasing concentrations of indicated laminarin. Graphs are one representative experiment of at least three replicates. {KD}

Product Details

PA5-34382

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A 17 amino acid synthetic peptide near the center of human CLEC7A
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
RRID	AB_2551734

Product Specific Information

A suggested positive control is rat spleen tissue lysate.

PA5-34382 can be used with blocking peptide PEP-1425.

Target Information

CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immunoregulatory Activity of the Natural Product Laminarin Varies Widely as a Result of Its Physical Properties.

Journal of immunology (Baltimore, Md. : 1950)

Smith AJ,Graves B,Child R,Rice PJ,Ma Z,Lowman DW,Ensley HE,Ryter KT,Evans JT,Williams DL

PA5-34382 was used in Western Blotting to show that laminarin can be either a Dectin-1 antagonist or agonist depending on the physiochemical properties, purity and structure of the laminarin preparation employed.

Mon Jan 15 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Beta-glucan receptor; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; CD369; DC-associated C-type lectin 1; dectin-1; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1

Gene Aliases: beta-GR; BGR; CANDF4; CD369; CLEC7A; Clec7b; CLECSF12; DECTIN1; RGD1565140; SCARE2; UNQ539/PRO1082

UniProt ID: (Human) Q9BXN2

Entrez Gene ID: (Human) 64581, (Mouse) 56644, (Rat) 502902

Function(s)