Immunofluorescent analysis of Dengue Virus Type 2 PrM showing staining in the cytoplasm of BHK-21 cells. BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH and stained using a Dengue Virus Type 2 PrM polyclonal antibody (Product # PA5-34967) diluted at 1:2000. Blue: Hoechst 33342 staining.
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 1 and 91 of PrM (Dengue virus 2)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34967 targets Dengue Virus PrM in WB and IF applications and shows reactivity with Virus samples.
The PA5-34967 immunogen is synthetic peptide corresponding to a region within amino acids 1 and 91 of PrM (Dengue virus 2).
Dengue fever and dengue hemorrhagic fever are acute febrile diseases, found in the tropics and Africa. Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. Dengue is transmitted to humans by the Aedes aegypti (rarely Aedes albopictus) mosquito, which feeds during the day.
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