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Immunofluorescent analysis of Desmin (Green) in primary human hepatic stellate cells. The cells were fixed with 1% buffered formaldehyde for 15 minutes, permeabilized with 0.1% TritonX-100 in DPBS for 15 minutes, and blocked with blocking buffer (1% BSA+10% Goat serum+0.3M Glycine in DPBS) for 1 hour at room temperature. Cells were stained overnight with anti-Desmin polyclonal antibody (Product # PA5-16705) at a dilution of 1:100 at 4oC and then incubated with alexa fluor 488 labeled secondary antibody (Product # A-11034) at a dilution of 1:1000 for 1 hour at room temperature. Cells were counterstained with ProLong® Gold antifade mountant with DAPI (Product # P36941) and imaged.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Sheep, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide mapping near the C-terminus of human desmin|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16705 targets Desmin in IHC (P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-16705 immunogen is a synthetic peptide mapping near the C-terminus of human desmin.
PA5-16705 has been successfully used in immunofluorescence and western blotting analysis of Desmin in primary human hepatic stellate cells.
Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.
PA5-16705 was used in immunohistochemistry - paraffin section to elucidate the origin of hepatocellular carcinoma.
|Mu X,Español-Suñer R,Mederacke I,Affò S,Manco R,Sempoux C,Lemaigre FP,Adili A,Yuan D,Weber A,Unger K,Heikenwälder M,Leclercq IA,Schwabe RF||The Journal of clinical investigation (125:3891)||2015|
Reconstruction of hepatic stellate cell-incorporated liver capillary structures in small hepatocyte tri-culture using microporous membranes.
PA5-16705 was used in immunohistochemistry to study the use of a thin, highly porous membrane to reconstruct hepatic stellate cell-incorporated liver capillary structures in an in vitro triculture
|Kasuya J,Sudo R,Masuda G,Mitaka T,Ikeda M,Tanishita K||Journal of tissue engineering and regenerative medicine (9:247)||2015|
Anti-interleukin-6 receptor antibody (MR16-1) promotes muscle regeneration via modulation of gene expressions in infiltrated macrophages.
PA5-16705 was used in immunohistochemistry to study the role of altered infiltrated macrophage gene expression in the mechanism by which an anti-IL-6 antibody promotes the regeneration of damaged skeletal muscle
|Fujita R,Kawano F,Ohira T,Nakai N,Shibaguchi T,Nishimoto N,Ohira Y||Biochimica et biophysica acta (1840:3170)||2014|
Diagnostic pitfalls of differentiating desmoplastic small round cell tumor (DSRCT) from Wilms tumor (WT): overlapping morphologic and immunohistochemical features.
PA5-16705 was used in immunohistochemistry to study the difficulty in differentially diagnosing desmoplastic small round cell tumor and Wilms tumor
|Arnold MA,Schoenfield L,Limketkai BN,Arnold CA||The American journal of surgical pathology (38:1220)||2014|
Endothelial-specific Notch blockade inhibits vascular function and tumor growth through an eNOS-dependent mechanism.
PA5-16705 was used in immunohistochemistry to study the role of eNOS in the mechanism by which vascular function and tumor growth are impaired following endothelial-specific inhibition of Notch
|Patenaude A,Fuller M,Chang L,Wong F,Paliouras G,Shaw R,Kyle AH,Umlandt P,Baker JH,Diaz E,Tong J,Minchinton AI,Karsan A||Cancer research (74:2402)||2014|
Solitary tumours associated with Jaagsiekte retrovirus in sheep are heterogeneous and contain cells expressing markers identifying progenitor cells in lung repair.
PA5-16705 was used in immunohistochemistry to study the heterogeneity and the presence of lung repair progenitor cells in Jaagsiekte retrovirus-associated solitary tumors in sheep
|De las Heras M,de Martino A,Borobia M,Ortín A,Álvarez R,Borderías L,Giménez-Más JA||Journal of comparative pathology (150:138)||2014|
Midkine-deficient mice delayed degeneration and regeneration after skeletal muscle injury.
PA5-16705 was used in immunohistochemistry to study the mechanisms underlying the retarded degeneration and regeneration observed following skeletal muscle injury in midkine ablated mice
|Ikutomo M,Sakakima H,Matsuda F,Yoshida Y||Acta histochemica (116:319)||2014|
Netrin-4 promotes mural cell adhesion and recruitment to endothelial cells.
PA5-16705 was used in immunohistochemistry to study the recruitment of mural cells to endothelial cells and the role played by Netrin-4
|Lejmi E,Bouras I,Camelo S,Roumieux M,Minet N,Leré-Déan C,Merkulova-Rainon T,Autret G,Vayssettes C,Clement O,Plouët J,Leconte L||Vascular cell (6:null)||2014|
Citral is renoprotective for focal segmental glomerulosclerosis by inhibiting oxidative stress and apoptosis and activating Nrf2 pathway in mice.
PA5-16705 was used in immunohistochemistry to study the roles of Nrf2 pathway activation and reduced levels of oxidative stress and apoptosis in the ability of citral to protect against focal segmental glomerulosclerosis in a murine model
|Yang SM,Hua KF,Lin YC,Chen A,Chang JM,Kuoping Chao L,Ho CL,Ka SM||PloS one (8:null)||2013|
Evidence for a common progenitor of epithelial and mesenchymal components of the liver.
PA5-16705 was used in immunohistochemistry to study whether hepatic epithelial and mesenchymal cells derive from a common progenitor
|Conigliaro A,Amicone L,Costa V,De Santis Puzzonia M,Mancone C,Sacchetti B,Cicchini C,Garibaldi F,Brenner DA,Kisseleva T,Bianco P,Tripodi M||Cell death and differentiation (20:1116)||2013|
The impact of KRAS mutations on VEGF-A production and tumour vascular network.
PA5-16705 was used in immunohistochemistry to study the differential effects on the production of VEGF-A and tumor vasculature development of codon 12 and codon 13 KRAS mutations
|Figueras A,Arbos MA,Quiles MT,Viñals F,Germà JR,Capellà G||BMC cancer (13:null)||2013|
Analysis of transplanted bone marrow-derived cells in chronic pancreatitis.
PA5-16705 was used in immunohistochemistry to study the transplantation of bone marrow-derived cells in a murine model of acute pancreatitis
|Westphalen CB,Marrache F,Wang TC||Methods in molecular biology (Clifton, N.J.) (980:291)||2013|
Oral administration of recombinant adeno-associated virus-mediated bone morphogenetic protein-7 suppresses CCl(4)-induced hepatic fibrosis in mice.
PA5-16705 was used in immunohistochemistry to study the ability of an orally administered recombinant AAV-BMP-7 virus to protect against experimentally induced hepatic fibrosis in a murine model
|Hao ZM,Cai M,Lv YF,Huang YH,Li HH||Molecular therapy : the journal of the American Society of Gene Therapy (20:2043)||2012|
Alterations in intermediate filaments expression in disc cells from the rat temporomandibular joint following exposure to continuous compressive force.
PA5-16705 was used in immunohistochemistry to study the effects of continuous compressive force on rat temporomandibular joint disc cell intermediate filaments expression
|Magara J,Nozawa-Inoue K,Suzuki A,Kawano Y,Ono K,Nomura S,Maeda T||Journal of anatomy (220:612)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
PA5-16705 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Lymphoid tissue damage in HIV-1 infection depletes naïve T cells and limits T cell reconstitution after antiretroviral therapy.
PA5-16705 was used in immunohistochemistry to test if damage to the lymphoid tissue fibroblastic reticular cell network contributes to naive T cell loss during HIV-1 infection
|Zeng M,Southern PJ,Reilly CS,Beilman GJ,Chipman JG,Schacker TW,Haase AT||PLoS pathogens (8:null)||2012|
Mesodermal mesenchymal cells give rise to myofibroblasts, but not epithelial cells, in mouse liver injury.
PA5-16705 was used in immunocytochemistry and immunohistochemistry to study the ability of murine mesodermal mesenchymal cells to differentiate into myofibroblasts but not epithelial cells
|Lua I,James D,Wang J,Wang KS,Asahina K||Hepatology (Baltimore, Md.) (60:311)||2014|
Downregulation of microRNA-9 in iPSC-derived neurons of FTD/ALS patients with TDP-43 mutations.
PA5-16705 was used in immunocytochemistry to use patient induced pluripotent stem cell-derived neurons to study the decreased miRNA-9 levels in frontotemporal dementia/ALS patients bearing mutations of TDP-43
|Zhang Z,Almeida S,Lu Y,Nishimura AL,Peng L,Sun D,Wu B,Karydas AM,Tartaglia MC,Fong JC,Miller BL,Farese RV,Moore MJ,Shaw CE,Gao FB||PloS one (8:null)||2013|
Modeling key pathological features of frontotemporal dementia with C9ORF72 repeat expansion in iPSC-derived human neurons.
PA5-16705 was used in immunocytochemistry to use patient induced pluripotent stem cell-derived neurons to explore the pathophysiology of C9ORF72 repeat expansion frontotemporal dementia
|Almeida S,Gascon E,Tran H,Chou HJ,Gendron TF,Degroot S,Tapper AR,Sellier C,Charlet-Berguerand N,Karydas A,Seeley WW,Boxer AL,Petrucelli L,Miller BL,Gao FB||Acta neuropathologica (126:385)||2013|
Lactic acid bacteria convert human fibroblasts to multipotent cells.
PA5-16705 was used in immunocytochemistry to study the ability of gut lactic acid bacteria to generate multipotent cells from human dermal fibroblasts
|Ohta K,Kawano R,Ito N||PloS one (7:null)||2013|
Induced pluripotent stem cell models of progranulin-deficient frontotemporal dementia uncover specific reversible neuronal defects.
PA5-16705 was used in immunocytochemistry to study reversible neuronal defects in frontotemporal dementia using induced pluripotent stem cell models
|Almeida S,Zhang Z,Coppola G,Mao W,Futai K,Karydas A,Geschwind MD,Tartaglia MC,Gao F,Gianni D,Sena-Esteves M,Geschwind DH,Miller BL,Farese RV,Gao FB||Cell reports (2:789)||2012|
|Not Applicable||Not Cited||
Gene expression during normal and FSHD myogenesis.
PA5-16705 was used in immunocytochemistry to study gene expression during normal and facioscapulohumeral muscular dystrophy myogenesis
|Tsumagari K,Chang SC,Lacey M,Baribault C,Chittur SV,Sowden J,Tawil R,Crawford GE,Ehrlich M||BMC medical genomics (4:null)||2011|
Fate tracing reveals hepatic stellate cells as dominant contributors to liver fibrosis independent of its aetiology.
PA5-16705 was used in immunohistochemistry and western blot to use a novel transgenic mouse to study the dominant role of hepatic stellate cells in liver fibrosis in liver disease regardless of its cause
|Mederacke I,Hsu CC,Troeger JS,Huebener P,Mu X,Dapito DH,Pradere JP,Schwabe RF||Nature communications (4:null)||2013|
HSCs play a distinct role in different phases of oval cell-mediated liver regeneration.
PA5-16705 was used in western blot to study the role of hepatic stellate cells at various time-points during liver regeneration mediated by hepatic oval cells
|Chen L,Zhang W,Zhou QD,Yang HQ,Liang HF,Zhang BX,Long X,Chen XP||Cell biochemistry and function (30:588)||2012|
Desmin; intermediate filament protein; mutant desmin p.K241E
CSM1; CSM2; DES; LGMD2R