Invitrogen

Desmoplakin Polyclonal Antibody

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Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver injury using DSP Polyclonal antibody (Product # PA5-96741) at a dilution of 1:100 (40x lens).

Desmoplakin Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using DSP Polyclonal antibody (Product # PA5-96741) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:200

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DSP.

Conjugate

Unconjugated

Form

Liquid

Concentration

2.96 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: MSCNGGSHPR INTLGRMIRA ESGPDLRYEV TSGGGGTSRM YYSRRGVITD QNSDGYCQTG TMSRHQNQNT IQELLQNCSD CLMRAELIVQ PELKYGDGIQ; Positive Samples: A-431, Mouse heart; Cellular Location: Cell junction, Cytoplasm, cytoskeleton, desmosome

Target Information

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.