Invitrogen

Desmoplakin Polyclonal Antibody

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Desmoplakin Antibody in Western Blot (WB) — Western Blot analysis of Desmoplakin in extracts of various cell lines using Desmoplakin Polyclonal Antibody (Product # PA5-96741) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Desmoplakin Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of Desmoplakin in paraffin-embedded Human tonsil using Desmoplakin Polyclonal Antibody (Product # PA5-96741) at a dilution of 1:100.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500 - 1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Desmoplakin

Conjugate

Unconjugated

Form

Liquid

Concentration

3.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2808543

Product Specific Information

Immunogen sequence: MSCNGGSHPR INTLGRMIRA ESGPDLRYEV TSGGGGTSRM YYSRRGVITD QNSDGYCQTG TMSRHQNQNT IQELLQNCSD CLMRAELIVQ PELKYGDGIQ; Positive Samples: A-431, Mouse heart; Cellular Location: Cell junction, Cytoplasm, cytoskeleton, desmosome

Target Information

Desmoplakin (DSP) is a major high molecular weight protein of the desmosomes. It is involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. Desmoplakin mutations are associated with Keratoderma and cardiomyopathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 250/210 kDa paraneoplastic pemphigus antigen; Desmoplakin; desmoplakin I/II; Desmoplakin II; Desmoplakin-I; desmosomal cytoskeletal connector molecule; DP; Oculoglycan; Opticin

Gene Aliases: 2300002E22Rik; 5730453H04Rik; AA407887; AA407888; AW109828; DCWHKTA; DP; DSP; OPT; OPTC; rul

UniProt ID: (Human) P15924, (Mouse) E9Q557, (Human) Q9UBM4, (Mouse) Q920A0

Entrez Gene ID: (Human) 1832, (Mouse) 109620, (Rat) 306871, (Human) 26254, (Mouse) 269120, (Rat) 304802

Molecular Function: actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein extracellular matrix protein receptor