|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 201-230 amino acids from the N-terminal region of mouse Dnmt2|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
mDnmt2 does not appear to be active as a DNA methyltransferase; however, its strong binding to DNA suggests that it may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. mDnmt2 is strongly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells. This protein belongs to the 5-cystosine methyltransferase family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Curcumin induces oxidation-dependent cell cycle arrest mediated by SIRT7 inhibition of rDNA transcription in human aortic smooth muscle cells.
PA5-11187 was used in immunocytochemistry and western blot to determine if curcumin can diminish/prevent the development of cardiovascular pathologies
|Lewinska A,Wnuk M,Grabowska W,Zabek T,Semik E,Sikora E,Bielak-Zmijewska A||Toxicology letters (233:227)||2015|