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Identifyn
F(ab')2-Donkey anti-Rabbit IgG, (H+L) SRM Identifyn™ Secondary Antibody, Alexa Fluor™ 405
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FIGURE: 1 / 16
Rabbit IgG, (H+L) SRM Secondary Antibody (SA000010) in ICC/IF
IdentifynTM Detailed protocols can be found below under Documents & Downloads. Confocal Microscopy: HeLa Cells were grown on acid-etched 1.5H cover glass to ~50% confluency, followed by -20°C methanol fixation and blocked with Thermo Fisher, Fish Serum Blocker (Product # 37527X3). The first primary antibody, Actin Recombinant Rabbit Monoclonal Antibody (JF47-01), was incubated overnight at 4°C at a dilution... View More
Product Details
SA000010
Applications
Tested Dilution
Western Blot (WB)
1:2,000
Immunocytochemistry (ICC/IF)
1:250-1:1,000
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Donkey
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Proprietary Immunogen.
Conjugate
Excitation/Emission Max
401/422 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 10% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target
IgG
Antibody Form
F(ab')2 Fragment
Product Specific Information
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:1,000
Widefield SIM - 1:1,000
Confocal - 1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) - 1:1,000
Detailed protocols can be found below under Methods & Protocols.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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