This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View Details
PA1-660 detects dynamin I (Dyn1) from rat tissues.
PA1-660 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn1 from rat whole brain extract.
PA1-660 immunizing peptide corresponds to amino acid residues 633-647 from rat Dyn1. This sequence is completely conserved between rat, mouse, and human Dyn1. PA1-660 immunizing peptide (Cat. # PEP-042) is available for use in neutralization and control experiments.
Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-dynamin; D100; Dyn1; Dynamin BREDNM19; dynamin i; Dynamin, brain; Dynamin-1; Dynamin1; EC 18.104.22.168; Neuronal dynamin; OTTHUMP00000215500
Gene Aliases: AI838169; DNM; DNM1; EIEE31; Ftfl; Kiaa4093; mKIAA4093
Molecular Function: membrane traffic protein