PA1-661 detects dynamin II (Dyn2) from rat, mouse, and human tissues and cells.
PA1-661 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn2 from HeLa cell lysate.
PA1-661 immunizing peptide corresponds to amino acid residues 760-779 from human Dyn2. This sequence is 95% conserved in mouse and rat Dyn2. PA1-661 immunizing peptide (Cat. # PEP-056) is available for use in neutralization and control experiments.
The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin I is expressed exclusively in neurons, dynamin II is ubiquitously expressed, and dynamin III is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dyn2; dynamin II; Dynamin UDNM; Dynamin-2
Gene Aliases: b2b2159Clo; CMT2M; CMTDI1; CMTDIB; DI-CMTB; DNM2; DYIIAAB; DYN2; DYNII; LCCS5; Udnm