Immunofluorescent staining of HeLa cells using PA5-19800, anti-Dynamin 2 antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 1 ug/ml overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Dynamin 2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with cow, Xenopus laevis and zebrafish based on sequence homology.
Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers.
PA5-19800 was used in western blot to study slow-twitch type 1 muscle fibers and diaphragm assessment in mice overexpressing phospholamban
|Fajardo VA,Smith IC,Bombardier E,Chambers PJ,Quadrilatero J,Tupling AR||Brain and behavior (6:null)||2016|
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.
PA5-19800 was used in western blot to study SERCA dysfunction in mice overexpressing phospholamban
|Fajardo VA,Bombardier E,McMillan E,Tran K,Wadsworth BJ,Gamu D,Hopf A,Vigna C,Smith IC,Bellissimo C,Michel RN,Tarnopolsky MA,Quadrilatero J,Tupling AR||Disease models and mechanisms (8:999)||2015|
Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression.
PA5-19800 was used in western blot to study the role of Rac1 in IL-1 receptor internalization and IL-1-induced gene expression
|Windheim M,Hansen B||Cellular signalling (26:49)||2014|