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Invitrogen
E2F6 Polyclonal Antibody
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FIGURE: 1 / 1
E2F6 Antibody (PA5-95059) in WB
Western blot analysis of E2F6 in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: COLO320 whole cell lysate (40 µg), Lane 3: A549 whole cell lysate (40 µg), Lane 4: MCF-7 whole cell lysate (40 µg), Lane 5: SMMC whole cell lysate (40 µg). Samples were incubated with E2F6 polyclonal antibody (Product # PA5-95059).
Product Details
PA5-95059
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human E2F6 (162-177aa KDCAQQLFELTDDKEN).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: HELA whole cell, COLO320 whole cell, A549 whole cell, MCF-7 whole cell, SMMC whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2F-6; E2F-binding site-modulating activity protein; E2F6-129; E2F6-206; E2F6-249; E2F6-281; EMA; MGC111545; new member of E2F transcription factor family; Transcription factor E2F6; transcriptional repressor E2F6; unnamed protein product
Gene Aliases: AI462434; E2F-6; E2F6; E2F6b; EMA
UniProt ID: (Human) O75461, (Mouse) O54917
Entrez Gene ID: (Human) 1876, (Mouse) 50496, (Rat) 313978
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
protein dimerization activity
RNA metabolism protein
nucleic acid metabolism protein
general transcription factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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