|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding amino acids residues 1-851 of human E6-AP expressed in E. coli.|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-23212 detects E6AP in Human samples. Customer testing reported no specific cross reactivity with Drosophila melanogaster E6AP.
PA1-23212 has been successfully used in Western Blot procedures.
PA1-23212 immunogen corresponds to Recombinant protein encoding amino acids residues 1-851 of human E6-AP expressed in E. coli..
The E6-associated protein (E6-AP/UBE3A), a 100a protein, was originally identified as an ubiquitin-protein ligase (E3), in the presence of the E6 protein from human papillomavirus types 16 and 18. E6-AP has also been shown to function as a coactivator of steroid receptor-dependent transcriptional activity. Recent data indicate that the ubiquitin-protein ligase function of E6-AP is dispensable for its ability to coactivate nuclear hormone receptors, showing that E6-AP possesses two separable independent functions, as both a coactivator and a ubiquitin-protein ligase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.