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Invitrogen
E6AP Polyclonal Antibody
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Product Details
PA3-843
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full-length human E6-AP:GST fusion protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-843 detects Angelman Syndrome-Associated protein (E6-AP) from human tissues.
PA3-843 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate.
IMPORTANT: E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.
PA3-843 antigen is bacterially expressed, full-length human E6-AP.
Target Information
UBE3A (ubiquitin-protein ligase E3A, AKA E6AP) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. UBE3A also plays an important role in the regulation of the circadian clock. Defects in UBE3A are a cause of Angelman syndrome (AS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; FLJ26981; HECT-type ubiquitin transferase E3A; human papilloma virus E6-associated protein; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; Ubiquitin-protein ligase E3A
Gene Aliases: ANCR; AS; E6-AP; E6AP; EPVE6AP; HPVE6A; UBE3A
UniProt ID: (Human) Q05086
Entrez Gene ID: (Human) 7337
ovarian follicle development
proteolysis
ubiquitin-dependent protein catabolic process
brain development
positive regulation of phosphatidylinositol 3-kinase signaling
viral process
androgen receptor signaling pathway
positive regulation of protein ubiquitination
sperm entry
regulation of circadian rhythm
positive regulation of transcription from RNA polymerase II promoter
protein autoubiquitination
prostate gland growth
protein K48-linked ubiquitination
regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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