Search
Invitrogen
E6AP Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Product Details
PA3-843
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full-length human E6-AP:GST fusion protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-843 detects Angelman Syndrome-Associated protein (E6-AP) from human tissues.
PA3-843 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate.
IMPORTANT: E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.
PA3-843 antigen is bacterially expressed, full-length human E6-AP.
Target Information
UBE3A (ubiquitin-protein ligase E3A, AKA E6AP) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. UBE3A also plays an important role in the regulation of the circadian clock. Defects in UBE3A are a cause of Angelman syndrome (AS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CTCL tumor antigen se37-2; E6-AP; E6AP ubiquitin-protein ligase; FLJ26981; HECT-type ubiquitin transferase E3A; human papilloma virus E6-associated protein; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; Ubiquitin-protein ligase E3A; unnamed protein product
Gene Aliases: ANCR; AS; E6-AP; E6AP; EPVE6AP; HPVE6A; PIX1; UBE3A
UniProt ID: (Human) Q05086
Entrez Gene ID: (Human) 7337
protein polyubiquitination
ovarian follicle development
proteolysis
ubiquitin-dependent protein catabolic process
brain development
positive regulation of macromolecule metabolic process
protein ubiquitination
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
positive regulation of protein ubiquitination
cellular response to nutrient levels
response to progesterone
sperm entry
regulation of circadian rhythm
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of transcription from RNA polymerase II promoter
regulation of synaptic plasticity
rhythmic process
animal organ development
system development
modulation of synaptic transmission
progesterone receptor signaling pathway
protein autoubiquitination
positive regulation of protein kinase B signaling
prostate gland growth
protein K48-linked ubiquitination
regulation of primary metabolic process
positive regulation of protein localization to lysosome
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
positive regulation of Golgi lumen acidification
regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support