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Invitrogen

E6AP Polyclonal Antibody

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E6AP Polyclonal Antibody

Product Details

PA5-95918

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human UBE3A (NP_5708531)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Amount

13 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807720

Product Specific Information

Immunogen sequence: KLIRLWSKYN ADQIRRMMET FQQLITYKVI SNEFNSRNLV NDDDAIVAAS KCLKMVYYAN VVGGEVDTNH NEEDDEEPIP ESSELTLQEL LGEERRNKKG PRVDPLETEL GVKTLDCRKP LIPFEEFINE PLNEVLEMDK DYTFFKVETE NKFSFMTCPF ILNAVTKNLG LYYDNRIRMY SERRITVLYS LVQGQQLNPY LRLKVRRDHI IDDALVRLEM IAMENPADLK KQLYVEFEGE QGVDEGGVSK EFFQLVVEEI FNPDIGMFTY DESTKLFWFN PSSFETEGQF T; Positive Samples: 293T, SKOV3, BT-474, U-251MG, Jurkat, Mouse brain, Mouse heart, Mouse thymus, Rat brain; Cellular Location: Cytoplasm, Nucleus

Target Information

UBE3A (ubiquitin-protein ligase E3A, AKA E6AP) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. UBE3A also plays an important role in the regulation of the circadian clock. Defects in UBE3A are a cause of Angelman syndrome (AS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CTCL tumor antigen se37-2; E6-AP; E6-AP ubiquitin protein ligase; E6AP ubiquitin-protein ligase; FLJ26981; HECT-type ubiquitin transferase E3A; human papilloma virus E6-associated protein; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; ubiquitin conjugating enzyme E3A; Ubiquitin-protein ligase E3A; unnamed protein product

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Gene Aliases: 4732496B02; 5830462N02Rik; A130086L21Rik; ANCR; AS; E6-AP; E6AP; EPVE6AP; HPVE6A; mKIAA4216; PIX1; UBE3A

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UniProt ID: (Human) Q05086, (Mouse) O08759

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Entrez Gene ID: (Human) 7337, (Mouse) 22215

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Function(s)
transcription coactivator activity ubiquitin-protein transferase activity protein binding zinc ion binding transferase activity metal ion binding ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase
Process(es)
protein polyubiquitination ovarian follicle development proteolysis ubiquitin-dependent protein catabolic process brain development positive regulation of macromolecule metabolic process protein ubiquitination intracellular steroid hormone receptor signaling pathway androgen receptor signaling pathway positive regulation of protein ubiquitination cellular response to nutrient levels response to progesterone sperm entry regulation of circadian rhythm proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of transcription from RNA polymerase II promoter regulation of synaptic plasticity rhythmic process animal organ development system development modulation of synaptic transmission progesterone receptor signaling pathway protein autoubiquitination positive regulation of protein kinase B signaling prostate gland growth protein K48-linked ubiquitination regulation of primary metabolic process positive regulation of protein localization to lysosome negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of Golgi lumen acidification regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of phosphatidylinositol 3-kinase signaling protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of nucleic acid-templated transcription
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