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Invitrogen
EBF2 Polyclonal Antibody
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FIGURE: 1 / 3
EBF2 Antibody (PA5-47890) in IHC (F)
Immunohistochemical analysis of EBF2 in immersion fixed frozen sections of mouse embryo (15 d.p.c.). Samples were incubated in EBF2 polyclonal antibody (Product # PA5-47890) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells.
Product Details
PA5-47890
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse EBF-2 Arg407-Ser519
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) EBF-1 and rmEBF-3 is observed.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
Early B-cell factor 2 (EBF2) is a gene located on chromosome 8p11.23, encoding a transcription factor involved in the regulation of gene expression during neural and adipose tissue development. EBF2 belongs to the COE family of transcription factors and plays a pivotal role in cellular differentiation processes. In the nervous system, EBF2 is essential for the development and function of neurons, particularly in regions like the olfactory system. Additionally, EBF2 is involved in adipogenesis, where it regulates genes responsible for the differentiation of brown adipocytes, contributing to thermogenesis and energy metabolism. Dysfunction or altered expression of EBF2 can impact neurological function and metabolic homeostasis, potentially influencing conditions such as obesity, diabetes, and neurodevelopmental disorders. Research into EBF2 is exploring its role in these physiological contexts and the implications of its regulatory mechanisms, offering insights into potential therapeutic approaches.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Collier, Olf and EBF 2; Collier, Olf and EBF transcription factor 2; early B cell factor 2; Early B-cell factor 2; EBF-2; MET-mesencephalon-olfactory TF1; MET-mesencephalon-olfactory transcription factor 1; metencephalon-mesencephalnon-olfactory transcription factor 1; Metencephalon-mesencephalon-olfactory transcription factor 1; O/E-3; OLF-1/EBF-LIKE 3; Transcription factor COE2; unnamed protein product
Gene Aliases: COE2; D14Ggc1e; EBF-2; EBF2; Mmot1; O/E-3; OE-3
UniProt ID: (Human) Q9HAK2, (Mouse) O08792
Entrez Gene ID: (Human) 64641, (Mouse) 13592
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
metal ion binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein dimerization activity
DNA-binding transcription factor
cell fate determination
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
brown fat cell differentiation
adipose tissue development
positive regulation of cold-induced thermogenesis
transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
positive regulation of chromatin binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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